Week 8 (Reporter Genes)
- Reporter gene
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ß-glucuronidase (GUS) (E.coli)
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green fluorescent protein (GFP) (jellyfish)
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luciferase (firefly)
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Inducible expression
-
Nucleic acid probe hybridization
Reading List
- Brown T. A., Gene Cloning and DNA Manipulation- An Introduction (2006) 5th Ed., Blackwell Publishmg Inc., USA
- Prinrose S.B., Twyman R.M., and Old R.W. (2006) Principles of Gene Manipulation, 6th Ed., pp. 50-55.
- Tanley W.M.S., and Rapley, Molecular Biomethods Handbook, Chapter 16, Plasmid-Derived Cloning Vectors, Human Press Inc, Totowa. NJ pp, 165-179
Course Material
- Week 1 (Purification of DNA from living cells)
- Week 2 (DNA Isolation and Purification)
- Week 3 (Plasmid and Plant DNA preparations)
- Week 4 (Polymerase chain reaction I)
- Week 5 (Polymerase Chain Reaction II)
- Week 6 (Introduction of DNA into Living Cells)
- Week 7 (Identification of recombinants )
- Week 8 (Reporter Genes)
- Week 9 (Introduction of phage DNA into bacterial cells)
- Week 10 (Identification of recombinant phages)
- Week 11 (Introduction of DNA into non-bacterial cells)
- Week 12 (Cloning Vectors or E.coli)
- Week 13 (Cloning vectors based on bacteriophage)
- Week 14 (Cloning Vectors for Eukaryotes)
- Week 15 (Plant and Animal Cloning Vectors)
- Week 16 (The Applications of Gene Cloning I)
- Week 17 (Applications II - Gene Expression and Function)
- Week 18 (Applications of Gene Cloning and DNA Analysis in Biotechnology)
- Chapters 18
- Department Chemistry
- Teacher
Dr Muhammad Fayyaz ur Rehman
