Week 4 (Polymerase chain reaction I)

  • Polymerase chain reaction
  • History, reaction, Steps, factors, primer designing, Application of PCR
  • Conditions for pcr reactions
  • DNA Polymerase types and properties
  • Reverse Transcriptase

Reeading List 

  1. Gene Expression in Recombinant Microorganisms, A. Smith (1995), Marcel Dekker, New York.
  2. DNA fingerprinting, M. Krawezak and J. Schmidtke (1994), Bioscientific, Oxford
  3. Principles of Biochemistry, A. L. Lehninger, D. L. Nelson and M. M. Cox, 3rd Editions (2000), Worth Publishers, New York.