Week 17 (Applications II - Gene Expression and Function)

  • Gene Expression and Function
  • RNA transcript of a gene – RNA Seq
  • Transcript mapping by hybridization between gene and RNA
  • Transcript analysis by primer extension
  • Transcript analysis by PCR
  • Studying the regulation of gene expression
  • Identifying protein binding sites on a DNA molecule
  • Gel retardation of DNA–protein complexes
  • Footprinting with DNase I
  • Modification interference assays
  • Identifying control sequences by deletion analysis
  • Reporter genes

 

Reading List 

  1. Brown T. A., Gene Cloning and DNA Manipulation- An Introduction (2006) 5th Ed., Blackwell Publishmg Inc., USA
  2. Prinrose S.B., Twyman R.M., and Old R.W. (2006) Principles of Gene Manipulation, 6th Ed., pp. 50-55.
  3. Tanley W.M.S., and Rapley, Molecular Biomethods Handbook, Chapter 16, Plasmid-Derived Cloning Vectors, Human Press Inc, Totowa. NJ pp, 165-179