Week 11 (Introduction of DNA into non-bacterial cells)

  • Introduction of DNA into non-bacterial cells
  • Transformation of individual cells
    • liposomes
    • protoplasts
  • electroporation
  • Microinjection
  • Biolistics
  • Transformation of whole organisms
    • animals and plants

Reading List 

  1. Brown T. A., Gene Cloning and DNA Manipulation- An Introduction (2006) 5th Ed., Blackwell Publishmg Inc., USA
  2. Prinrose S.B., Twyman R.M., and Old R.W. (2006) Principles of Gene Manipulation, 6th Ed., pp. 50-55.
  3. Tanley W.M.S., and Rapley, Molecular Biomethods Handbook, Chapter 16, Plasmid-Derived Cloning Vectors, Human Press Inc, Totowa. NJ pp, 165-179
  4. Gene Expression in Recombinant Microorganisms, A. Smith (1995), Marcel Dekker, New York.