Week 5 (Polymerase Chain Reaction II)
- Variants of PCR
- Real time PCR, Q-PCR
- TaqMan Probe
- Molecular Beacons
- Inverse PCR
- LM-PCR
- Ligase chain reaction
Reading List
- Gene Expression in Recombinant Microorganisms, A. Smith (1995), Marcel Dekker, New York.
- DNA fingerprinting, M. Krawezak and J. Schmidtke (1994), Bioscientific, Oxford
- Principles of Biochemistry, A. L. Lehninger, D. L. Nelson and M. M. Cox, 3rd Editions (2000), Worth Publishers, New York.
Course Material
- Week 1 (Purification of DNA from living cells)
- Week 2 (DNA Isolation and Purification)
- Week 3 (Plasmid and Plant DNA preparations)
- Week 4 (Polymerase chain reaction I)
- Week 5 (Polymerase Chain Reaction II)
- Week 6 (Introduction of DNA into Living Cells)
- Week 7 (Identification of recombinants )
- Week 8 (Reporter Genes)
- Week 9 (Introduction of phage DNA into bacterial cells)
- Week 10 (Identification of recombinant phages)
- Week 11 (Introduction of DNA into non-bacterial cells)
- Week 12 (Cloning Vectors or E.coli)
- Week 13 (Cloning vectors based on bacteriophage)
- Week 14 (Cloning Vectors for Eukaryotes)
- Week 15 (Plant and Animal Cloning Vectors)
- Week 16 (The Applications of Gene Cloning I)
- Week 17 (Applications II - Gene Expression and Function)
- Week 18 (Applications of Gene Cloning and DNA Analysis in Biotechnology)
- Chapters 18
- Department Chemistry
- Teacher
Dr Muhammad Fayyaz ur Rehman
