Week 6 (Introduction of DNA into Living Cells)

  • Introduction of DNA into Living Cells
  • Purpose of cloning 
  • ligated, unligated and self-ligated vector identification 
  • Bacterial transformation 
  • Competent cells 
    • Preparation 
    • CaCl2 Method
    • BaCl2 Method 
  • Selection of Transformed Cells 

Reading List 

  1. Brown T. A., Gene Cloning and DNA Manipulation- An Introduction (2006) 5th Ed., Blackwell Publishmg Inc., USA
  2. Prinrose S.B., Twyman R.M., and Old R.W. (2006) Principles of Gene Manipulation, 6th Ed., pp. 50-55.