Week 12 (Cloning Vectors or E.coli)

  • Cloning Vectors or E.coli
  • simplest cloning vectors - Plamsid Vectors
  • Nomenclature of plasmid cloning vectors
    • pBR322
    • Properties of pBR322
    • pBR327
  • pUC8—a Lac selection plasmid
  • Cloning vectors 

Reading List 

  1. Brown T. A., Gene Cloning and DNA Manipulation- An Introduction (2006) 5th Ed., Blackwell Publishmg Inc., USA
  2. Prinrose S.B., Twyman R.M., and Old R.W. (2006) Principles of Gene Manipulation, 6th Ed., pp. 50-55.
  3. Tanley W.M.S., and Rapley, Molecular Biomethods Handbook, Chapter 16, Plasmid-Derived Cloning Vectors, Human Press Inc, Totowa. NJ pp, 165-179
  4. Gene Expression in Recombinant Microorganisms, A. Smith (1995), Marcel Dekker, New York.