Week 13 (Cloning vectors based on bacteriophage)

 

  • Cloning vectors based on M13 bacteriophage
  • Construct a phage cloning vector
  • Hybrid plasmid–M13 vectors
  • Insertion and replacement vectors 98
  • Cloning experiments with 2 insertion or replacement vectors
  • Long DNA fragments clone by a cosmid
  • Lambda and other high-capacity vectors enable genomic libraries
  • Vectors for other bacteria

Reading List 

  1. Brown T. A., Gene Cloning and DNA Manipulation- An Introduction (2006) 5th Ed., Blackwell Publishmg Inc., USA
  2. Prinrose S.B., Twyman R.M., and Old R.W. (2006) Principles of Gene Manipulation, 6th Ed., pp. 50-55.
  3. Tanley W.M.S., and Rapley, Molecular Biomethods Handbook, Chapter 16, Plasmid-Derived Cloning Vectors, Human Press Inc, Totowa. NJ pp, 165-179
  4. Gene Expression in Recombinant Microorganisms, A. Smith (1995), Marcel Dekker, New York.