Week 16 (The Applications of Gene Cloning I)

  • Sequencing Genes and Genomes
  • Methodology for DNA sequencing
  •  Chain termination DNA sequencing
  • DNA polymerases for sequencing
  • Pyrosequencing
  • Massively parallel pyrosequencing
  • Whole genome Sequencing
  • The shotgun genome sequencing
  • The Haemophilus influenzae genome sequencing project
  • The clone contig approach
  • Clone contig assembly by chromosome walking
  • Clone contig assembly by sequence tagged site content analysis
  • Genetic maps

Reading List 

  1. Brown T. A., Gene Cloning and DNA Manipulation- An Introduction (2006) 5th Ed., Blackwell Publishmg Inc., USA
  2. Prinrose S.B., Twyman R.M., and Old R.W. (2006) Principles of Gene Manipulation, 6th Ed., pp. 50-55.
  3. Tanley W.M.S., and Rapley, Molecular Biomethods Handbook, Chapter 16, Plasmid-Derived Cloning Vectors, Human Press Inc, Totowa. NJ pp, 165-179