Week 1 (Purification of DNA from living cells)

  • Isolation of DNA
    • Total Cell DNA,
    • Plasmid DNA,
    • Phage DNA 
  • Media for bacterial DNA Isolation 
  • Defined Media 
  • Undefined Media
  • Growing and Harveting bacteria 
  • Bacetrial Lysis
  • Lysate Preparatio

Reading List 

  1. Brown T. A., Gene Cloning and DNA Manipulation- An Introduction (2006) 5th Ed., Blackwell Publishmg Inc., USA
  2. Prinrose S.B., Twyman R.M., and Old R.W. (2006) Principles of Gene Manipulation, 6th Ed., pp. 50-55.