Week 14 (Cloning Vectors for Eukaryotes)

  • Cloning Vectors for Eukaryotes
  • Vectors for yeast and other fungi
  • yeast episomal plasmids
  • Other types of yeast cloning vector
  • Artificial chromosomes in yeast
  • The structure and use of a YAC vector
  • Applications for YAC vectors
  • Vectors for other yeasts and fungi
  • Cloning vectors for higher plants
  • Agrobacterium tumefaciens— Ti plasmid

 

Reading List 

  1. Brown T. A., Gene Cloning and DNA Manipulation- An Introduction (2006) 5th Ed., Blackwell Publishmg Inc., USA
  2. Prinrose S.B., Twyman R.M., and Old R.W. (2006) Principles of Gene Manipulation, 6th Ed., pp. 50-55.
  3. Tanley W.M.S., and Rapley, Molecular Biomethods Handbook, Chapter 16, Plasmid-Derived Cloning Vectors, Human Press Inc, Totowa. NJ pp, 165-179
  4. Gene Expression in Recombinant Microorganisms, A. Smith (1995), Marcel Dekker, New York.