ANSC-6113                         Introductory Molecular Genetics                                              2(1-1)

ABG-303                          Introductory Molecular Genetics                                              2(1-1)

 

This is the introductory course of under graduate level involved the study of molecular genetics. Molecular genetics is the field of biology that studies the structure and function of genes at a molecular level and thus employs methods of both molecular biology and genetics. The study of chromosomes and gene expression of an organism can give insight into heredity, genetic variation, and mutations. This is useful in the study of developmental biology and in understanding and treating genetic diseases. Students will build a comprehensive working knowledge of proteins - their chemistry and their role as enzymes, structural, recognition, and signaling molecules, nucleic acids - their structures, properties, and their roles in the storage and transmission of genetic information. The roles of biomolecules in cellular signaling, metabolism, structure, and cellular organization the thermodynamics of specific molecular transformations, as well as integrated complex processes, and  the integration, interaction, and regulation of complex developmental, signaling, and metabolic pathways at the organism  level. The field of study is based on the merging of several sub-fields in biology:  classical Mendelian inheritance, cellular biology, molecular biology, biochemistry, and biotechnology.  Researchers search for mutations in a gene or induce mutations in a gene to link a gene sequence to a specific phenotype.  Molecular genetics is a powerful methodology for linking mutations to genetic conditions that may aid the search for treatments/cures for various genetics diseases.

 

Contents

Theory

  1. Biochemical basis of heredity: the nature of genetic material, nucleic acids
  2. Structure of DNA and RNA, DNA replication, Protein synthesis and its regulation.
  3. Developmental aspects of genetic control, Gene expression and cell differentiation
  4. Control of gene expression in eukaryotes, Genetic basis of immune response
  5. Components of immune system, immune response
  6. Genetic diversity, genetic diversity in immune system
  7. Mutations, gene mutations and their types
  8. Variation in chromosome structure and number
  9. Genetic engineering: basic concepts of recombinant DNA technology
  10. Gene cloning and manipulation, application and future.
  11. Causes of variation in gene expression.
  12. Phenotypic expression of gene, Bio chemical genetics.
  13. Concept of different types of genetics

 

Practical

  1. Demonstration of various cytogenetic techniques, Karyotyping and banding.
  2. DNA extraction, Gel electrophoresis.

 

Recommended Texts

  1. Hodge, R. (2009). Genetic Engineering: Manipulating the Mechanisms of Life.  New York, USA: infobase Publishing.
  2. Khan, F. H. & Singh, A. (2002). Principles of Genetics and Animal Breeding. India: JP Brothers Medical Publishers Ltd.

Suggested Books

  1. Lewis, R. (2005). Human Genetics. New York, USA: McGraw-Hill Co. Inc.
  2. Tamarin R. H. (1998). Principles of Genetics (6th ed.). USA: William C. Brown Pub.

COURSE SCHEDULE

Week

Topics and Readings:

Book Page No.

1

Demonstration of various cytogenetic techniques. Biochemical basis of heredity the nature of genetic material.  

Methods in Molecular Biology PCR Protocoal John M. S. Bartlett David Stirling.

 P,kankaraj A text book of Animal Genetics. Page No. 240-260

2

Demonstration of various cytogenetic techniques.  Methods in Molecular Biology PCR Protocoal John M. S. Bartlett David Stirling. nucleic acids, structure of DNA and RNA. Principles of genetics by Tamarin

(Page No. 245-258).

 Methods in Molecular Biology PCR Protocoal John M. S. Bartlett David Stirling.

Principles of genetics by Tamarin

(Page No. 245-258).

3

Demonstration of various cytogenetic techniques.     

Methods in Molecular Biology PCR Protocoal John M. S. Bartlett David Stirling. Page No. 20-28        

4

DNA replication, Principles of genetics by Tamarin (Page No. 220-242), Demonstration of various cytogenetic techniques. Methods in Molecular Biology PCR Protocoal John M. S. Bartlett David Stirling     

Principles of genetics by Tamarin (Page No. 220-242), Methods in Molecular Biology PCR Protocoal John M. S. Bartlett David Stirling           

5

Demonstration of various cytogenetic techniques. Transcription and translation, Protein synthesis and its regulation.

Principles of genetics by Tamarin (Page No. 245-275) and  (Page No. 281-311).

Methods in Molecular Biology PCR Protocoal John M. S. Bartlett David Stirling

6

  karyotyping and banding. Developmental aspects of genetic control. Gene Expression and cell differenciation. Control of gene expression in eukaryotes

Methods in Molecular Biology PCR Protocoal Principles of genetics by Tamarin (Page No.281- 303). Principles of genetics by Tamarin (Page No. 465-468).John M. S. Bartlett David Stirling.

7

karyotyping and banding.  Genetic basis of immune response: components of immune system. immune response, genetic diversity in immune system

Methods in Molecular Biology PCR Protocoal John M. S. Bartlett David Stirling. Principles of genetics by Tamarin (Page No. 492-494).

8

  karyotyping and banding.  Mutations, gene mutations and their types.

Methods in Molecular Biology PCR Protocoal John M. S. Bartlett David Stirling. A Text Book of Animal Genetics (P. Kanakaraj) (Page No. 257-266). 

9

 karyotyping and banding. variation in chromosome structure and number. Genetic engineering, DNA extraction and gel electrophoresis.

Methods in Molecular Biology PCR Protocoal Jo A Text Book of  Animal Genetics (P. Kanakaraj) (Page No. 251-258).hn M. S. Bartlett David Stirling.

10

karyotyping and banding. Genetic engineering, DNA extraction and gel electrophoresis.

Methods in Molecular Biology PCR Protocoal John M. S. Bartlett David Stirling. A Text Book of  Animal Genetics (P. Kanakaraj) (Page No. 251-258).

11

karyotyping and bandingMethods in. basic concepts of recombinant DNA technology.

Methods in Molecular Biology PCR Protocoal John M. S. Bartlett David Stirling. (Page No. 2-18)

12

DNA extraction and gel electrophoresis. gene cloning and manipulation, application and future. Phenotypic expression of gene.

Methods in Molecular Biology PCR Protocoal John M. S. Bartlett David Stirling. (Page No. 2-18)

13

Methods in Molecular Biology PCR Phenotypic expression of gene. Bio chemical genetics. Bio chemical genetics.

Methods in Molecular Biology PCR Protocoal John M. S. Bartlett David Stirling. (Page No. 2-18)

14

DNA extraction and gel electrophoresis Bio chemical genetics.(Page 417-426)

Methods in Molecular Biology PCR Protocoal John M. S. Bartlett David Stirling. Bio chemical genetics.(Page 417-426)

15

DNA extraction and gel electrophoresis. Concept of different types of genetics i.e cytogenetics, Molecular genetics, extranuclear genetics, population genetics, biochemical genetics, bacterial genetics, immunogentics, developmental genetics and applied genetics.

Methods in Molecular Biology PCR Protocoal John M. S. Bartlett David Stirling. (Page No. 2-18)

16

DNA extraction and gel electrophoresis. Concept of different types of genetics i.e cytogenetics, Molecular genetics, extranuclear genetics, population genetics, biochemical genetics, bacterial genetics, immunogentics, developmental genetics and applied genetics.

Methods in Molecular Biology PCR Protocoal John M. S. Bartlett David Stirling. (Page No. 2-18)

 

Sessional:    4

Project:         1

Presentation:     1

Participation:        2

Final Exam:                              6 Mid,

                                                   10 Theory

Course Material

Other Courses by Ms. Atia Iram