Course Title: Basic Microbiology

Course Code: Micro-202

Credit Hours: 3(2-1)

Instructor: Dr. Imtiaz Hussain       

Email: imtiaz.hussain@uos.edu.pk

 

DESCRIPTION & OBJECTIVES

Students will be able to identify important microbes of livestock and poultry. Can help in the prevention and control of important diseases of livestock in Pakistan

 

The key objectives/outcomes of this course are;

  • To impart basic and applied knowledge about microbes.

CONTENTS

Theory

Introduction to microbiology: Scope, definition, branches and applied areas of microbiology. Historical introduction including work of pasture, Koch, Lister and recent developments. Diversity of microbes. Differentiation between Prokaryotes and Eukaryotes. Taxonomy of bacteria: basis of taxonomy, taxonomy of bacteria, origin and evolution of bacteria, species concept in bacteria. Structure of Eubacteria: Slime layer, cell wall, protoplast, cytoplasmic membrane, cytoplasm, cytoplasmic inclusions and vacuoles, the nucleus, flagella, fimbrae, the bacterial endospores, cytoplasm. Microscopy: light, dark field, fluorescent, polarizing, phase contrast and special stains. A brief introduction to structure and propagation of fungi, protozoa, algae, viruses and bacteriophages.

Practical

Safety in the microbiological laboratory: contamination and decontamination. Demonstration of laboratory equipment, their basic functions and handling. Microscopy: An outline of principles and applications: Light, dark field, fluorescent, polarizing, phase contrast and electron microscopes. Use a bright field light microscope to view and interpret slides, including correctly setting up and focusing the microscope, proper handling, cleaning, and storage of the microscope, Correct use of all lenses, recording microscopic observations. Preparation of slides for microbiological examination including cleaning and disposing of slides, preparing smears from solid liquid cultures, performing wet mount and/or hanging drop preparations, performing Gram staining. Sterilization and disinfection: Physical agents including moist heat, dry heat, ionizing radiation, filtration etc. Chemical agents: antiseptics and disinfectants, evaluation of anti-microbial activity (Phenol coefficient). Methods of bacterial cultivation and growth, bacterial colonies: types and characteristics. Morphology: shape and arrangements, micrometery and motility.

 

READINGS

  • Collins, C.H., B.M. Lyne and J.M. Grange. 2000. Microbiological Methods. 8th Ed., Butter Worth Heinemann, Oxford .UK.
  • Jawetz, E. and W. Levinson. 2000. Medical Microbiology and Immunology. 5th Ed., Prentice Hall Ltd., London. UK.
  • Parker. M.T. and L.H. Collin. 1998. Topley and Wilson’s Principles of Bacteriology. Virology & Immunity. 9th Ed., Edward Arnold, London.UK.

 

COURSE SCHEDULE

Weeks

Topic and Readings

Books with Page No.

  1.  

Introduction to microbiology, Scope of microbiology, Safety in the microbiological laboratory

Veterinary Microbiology.

Page: 1-6.

16-01-2020

17-01-2020

2

Definition and branches of microbiology, Branches of microbiology, Contamination and decontamination.

Alcamo’s Fundamentals of Microbiology, 9 th edition. Page: 23. &

http://www.austincc.edu

23-01-2020

24-01-2020

3

Applied areas of microbiology, Historical introduction including work of pasture, Demonstration of laboratory equipments, their basic functions and handling.

Alcamo’s Fundamentals of Microbiology, 9 th edition. Page 15, 16 25, 26 & 27.

30-01-2020

31-01-2020

4

Historical introduction including work of pasture and Koch, Microscopy: An outline of principles and applications.

Alcamo’s Fundamentals of Microbiology, 9 th edition. Page:15-17.

6-02-2020

7-02-2020

5

Historical introduction including work of Lister and recent developments, Diversity of microbes, Differentiation between Prokaryotes and Eukaryotes, Light, dark field Microscopy, Fluorescent Microscopy.

Alcamo’s Fundamentals of Microbiology, 9 th edition. Page:15-17, 73-79, 83-91.

Bergeys manual of systematic bacteriology (second edition,2004)

13-02-2020

14-02-2020

6

Taxonomy, Polarizing Microscopy, phase contrast and electron microscopes.

Alcamo’s Fundamentals of Microbiology, 9 th edition. Page: 64-71, 83-91, 73-79 and 127.

 

20-02-2020

21-02-2020

7

Structure of Eubacteria: Slime layer, cell wall, Use a bright field light microscope to view and interpret slides, including correctly setting up and focusing the microscope.

Alcamo’s Fundamentals of Microbiology, 9 th edition. Page: 83-91, 91-124

 

27-02-2020

28-02-2020

8

Protoplast, cytoplasmic membrane, cytoplasm, cytoplasmic inclusions and vacuoles, the nucleus, flagella, fimbrae, the bacterial endospores, Proper handling, cleaning, and storage of the microscope, Correct use of all lenses, recording microscopic observations.

Alcamo’s Fundamentals of Microbiology, 9 th edition. Page: 83-91, 91-124

 

5-03-2020

6-03-2020

9

Mid Test

 

10

Taxonomy of bacteria, Preparation of slides for microbiological examination including cleaning and disposing of slides.

Alcamo’s Fundamentals of Microbiology, 9 th edition. Page: 73-79, 83-91.

19-03-2020

20-03-2020

11

Taxonomy of bacteria, Origin and evolution of bacteria, preparing smears from solid liquid cultures, performing wet mount and/or hanging drop preparations, performing Gram staining.

Alcamo’s Fundamentals of Microbiology, 9 th edition. Page: 73-79, 83-91.

26-03-2020

27-03-2020

12

Species concept in bacteria, Sterilization and disinfection: Physical agents including moist heat, dry heat, ionizing radiation, filtration, etc. chemical agents.

Alcamo’s Fundamentals of Microbiology, 9 th edition. Page:73-79, 191-200.

2-04-2020

3-04-2020

13

Microscopy, Light microscopy, Antiseptics and disinfectants.

Alcamo’s Fundamentals of Microbiology, 9 th edition. Page:83-91, 204-207.

9-04-2020

10-04-2020

14

Dark field microscopy, Fluorescent microscopy, Evaluation of anti-microbial activity (Phenol coefficient).

Alcamo’s Fundamentals of Microbiology, 9 th edition. Page:83-91, 204-207.

16-04-2020

17-04-2020

15

Polarizing microscopy, phase contrast and special stains, A brief introduction to structure and propagation of fungi, Methods of bacterial cultivation and growth,

Alcamo’s Fundamentals of Microbiology, 9 th edition. Page:83-91, 536-551

23-04-2020

24-04-2020

16

Polarizing microscopy, phase contrast and special stains, A brief introduction to structure and propagation of fungi, Methods of bacterial cultivation and growth,

Alcamo’s Fundamentals of Microbiology, 9 th edition. Page:83-91, 207, 536-551

30-04-2020

17

Final term

 

Note: You can reserve one week for sessional or mid-term exam, and if you wish, one week for student presentations of the assigned research project.

RESEARCH PROJECT

State here the prerequisites of the assigned research project, including term paper or lab assignment, etc.

 

ASSIGNMENT CRITERIA

Write here the distribution of the marks. You can choose any or all from below for the purpose.

Sessional: 08

Mid-term: 12

Final Exam: 20

Practical: 20

RUELS AND REGULATIONS

Following items are mandatory:

  • 80% class attendance.
  • Maintenance of practical notebook.
  • Participation in all practical performances and class activities.

Course Material