Practical Demonstration of Koch's Postulates for Fungi

•Presence of Pathogen

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–The suspected pathogen must be found associated with the disease in all the diseased plants examined.

•Isolation

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–The organism must be isolated from the diseased tissue and grown in pure culture on nutrient media

 

•Re-inoculation

–When a healthy plant, of the same species or variety, is inoculated with this culture, it must produce the disease and show the characteristic symptoms.

•Re-isolation

–The organism must be re-isolated from the inoculated plants and must be shown to be the same pathogen as the original.

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