This course deals with advanced techniques of research & industrial significance. The course provides comprehensive overview ranging from fundamental principles, instrumentation and applications of two major chromatographic techniques including gas liquid chromatography (GLC) and high performance liquid chromatography (HPLC) alongwith concept of theoretical plate model and efficiency of column etc. The course will also cover brief overview of recent advancements in these techniques. Theoretical principles and details about instrumentation of GLC and HPLC will be covered including sample preparation to sample injection, columns, detectors and integrators etc. The course encompasses applications of these techniques in different industries and quality control. The ultimate objective of this course is to impart knowledge of state-of-the-art chromatographic methods to students with specialization in analytical chemistry.

LEARNING OUTCOMES

Overall, the students will acquire knowledge and technical know-how about principles, instrumentation and applications of important chromatographic techniques such as GLC, HPLC alongwith recent advancements in these techniques. After acquiring the technical knowledge and successful accomplishment of this course, students will be able to apply these chromatographic techniques on practical grounds for qualitative and quantitative analysis of different chemical samples and characterization of various industrial and natural materials. These learnings will also assist students in the successful accomplishment of their research activities/projects at post graduate level.  

READINGSEADINGS

Recommended Books

1. Christian, G.D., Analytical Chemistry, 7th edition John Wiley & Sons, NY (2013).
2. Harris, D.C.  Quantitative Chemical Analysis, 9th Edition Freeman, NY (2016).
3. Skoog, D. A. West, D. Holler, J. M.F., and Crouch S. R. Fundamentals of Analytical Chemistry. 9th  ed. Saunders College Publishing, Philadelphia. (2014).

Suggested Books

1. Salvatore,  F., Paul R. Haddad , Colin Poole, David K. Lloyd. Liquid Chromatography: Fundamentals and Instrumentation (Handbooks in Separation Science) 1st Edition, Elsevier; (2013)
2. Nikalje, Anna. A Handbook of Chromatography, Scholar's Press Verlag Omniscriptam, Deutschland, Germany (2017)

COURSE CONTENTS

CONTENTS

Gas–Liquid chromatography, concepts of theoretical plates, Van – deemter equation, High–performance liquid chromatography, instrumentation and applications of these techniques.

COURSE SCHEDULECOURSE SCHEDULE

RESEA      RESEARCH PROJECT/ASSIGNMENTCH ASSIGNEMENT

The students will be given chromatography related assignments and they will prepare slides for presentation for training purposes.

ASSESSMENT CRITERIA RITERIA

Mid Term Examination: 30%

Sessional:  20%

Presentation: Included in sessional marks

Final Examination: 50%

RUL         RULES AND REGULATIONSES AND REGULATIONS

1. Minimum attendance 75% is necessary to appear in exam.
2. mobiles to be off/silent during class time and no permission of using mobile in exam
3. as per university academic policy