Advance Chromatographic Techniques- CHEM-413

This course deals with advanced techniques of research & industrial significance. The course provides comprehensive overview ranging from fundamental principles, instrumentation and applications of two major chromatographic techniques including gas liquid chromatography (GLC) and high performance liquid chromatography (HPLC) alongwith concept of theoretical plate model and efficiency of column etc. The course will also cover brief overview of recent advancements in these techniques. Theoretical principles and details about instrumentation of GLC and HPLC will be covered including sample preparation to sample injection, columns, detectors and integrators etc. The course encompasses applications of these techniques in different industries and quality control. The ultimate objective of this course is to impart knowledge of state-of-the-art chromatographic methods to students with specialization in analytical chemistry.

READINGS

Harris, D.C., Quantitative Chemical Analysis, W.H. Freeman & Company, New York, 2003.

Christian, G.D. Analytical Chemistry, John Wiley & Sons, NY, 2005

CONTENTS

COURSE SCHEDULE

Week	Topics and Readings
1.	Analytical Chemistry & Separation Techniques
2.	Solvent extraction; merits and demerits
3.	Fundamentals of Chromatography & Types
4.	Retention time, adjusted Rt, Capacity factor
5.	Partition coefficient, Scaling up of column
6.	Resolution, Efficiency of separation, Factors affecting resolution
7.	Plate height equation: Van Deemter equation; factors affecting H
8.	Types of columns, Effect of silanization on band broadening
9.	Mid Term Exam
10.	Gas chromatography: basic concepts
11.	Instrumentation
12.	Types of columns, and detectors
13.	Applications of Gas chromatography
14.	Liquid chromatography, Fundamentals and comparison with GC
15.	Detectors of Liquid chromatography
16.	Types of Elution chromatography
18	Final-Term Examination