Advance Spectroscopy I CHEM-412

The course introduces students to a study of basic principle of Spectroscopy, Its classifications and basic principle behind this technique. UV-Visb. Spectroscopy and Atomic spectroscopy will be discussed in in detail. We will study basic principle, instrumentation, spectral interpretation and applications of these techniques.

 

 

READINGS

 

  1. D.Skoog and D.M.West, Fundamentals of Analytical Chemistry, Holt Reinhart Inc, London
  2. G.D.Christian, Analytical Chemistry, John Wiley & Sons
  3. D.C. Harris , Quantitative Chemical Analysis, Freeman, N. Y.
  4. Undergraduate Instrumental Analysis by Robinson

 

CONTENTS

COURSE SCHEDULE

 

Week

Topics and Readings

1.

Introduction of Spectroscopy, Classification, Basic terminology

2.

Introduction of UV-Visb. Spectroscopy, types of transitions

3.

Instrumentation of UV-Visb. Spectrophotometer

4.

Woodward-Fieser Rule and its application on structure determination

5.

Determination of maximum wavelength of different compounds by applying Woodward-Fieser Rule

6.

Practice for determination of absorption maxima

7.

Applications of UV-Visb. Spectroscopy

8.

Flame Atomic Spectroscopy, Instrumentation ( source, flame types)

9.

Mid Term Exam

10.

Monochromator, detector, applications of the technique

11.

Graphite furnace AAS. Difference between FAAS and GFAAS

12.

Cold vapour AAS, Hydride generation, Interferences

13.

Flame emission spectroscopy, Instrumentation( source)

14.

Optical system, detector, applications

15.

Plasma emission spectroscopy, ICP, DCP

16.

applications of AES, electric emission spectroscopy Comparison of AAS, FES, PES, EES

17.

Test and problems

18

 
 

 

 

 

RESEARCH PROJECT

 

Analytical Chemistry Lab. (Cr.01)

 

  1. Measurement of λ-max and calculation of Molar absorptivity of potassium permanganate.

2. Plotting of calibration graph and measurement of unknown sample concentration.

3. Use of standard addition method in Spectrophotometry.

4. Determination of iron (II) using 1, 10-phenanthroline method.

5. Determination of iron (III) using thiocyanate method involving solvent extraction.

6. Determination of phosphate by Spectrophotometry using molybdenum blue method.

7. Determination of Sodium in tap water sample by using Flame photometer.

8. Determination of Potassium in tap water sample by using Flame photometer.

9. Determination of Calcium in chalk sample by using Flame photometer.

10. Determination of Calcium in drinking water by EDTA.

11. Identification of free salicylic acid in aspirin by using TLC.

12. Determination of Methylene blue value of activated charcoal. 

13. Determination of iron in tap water by AAS.

14. Determination of copper content in milk samples by AAS.

 

ASSESSMENT CRITERIA

Mid Term Exam: 15%

Sessional:     15%

Project/Practical work:       25%

Presentation:  included in sessional marks

Participation:  Nil

Final exam: 45%

RULES AND REGULATIONS

 

  1. Minimum attendance 75% is necessary to appear in exam.
  2. For practical exam also 75% attendance in practical is necessary.
  3. Keep your mobiles off/silent during class time.
 

Course Material

Other Courses by Dr. Fozia Batool