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Methods in Molecular Biology
Methods in Molecular Biology
Course Material
01
Solution dilutions
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02
Sterilization techniques
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03
DNA/RNA extraction techniques
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04
Analysis of nucleic acids by gel electrophoresis Horizontal, vertical, pulse field, denaturing gradient gel electrophoresis
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05
Analysis of proteins by native and SDS-PAGE; 2-D gels
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06
Polymerase chain reaction (PCR)
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07
Types of PCR (inverse, touch-down, nested, hemi-nested, pit stop
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08
Multiplex, reverse transcriptase, RACE
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09
Real-time qPCR
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10
Applications of PCR
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11
Detection of mutations and/or SNPs
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12
Detection of mutations and/or SNPs
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13
Enzyme-linked immunosorbant assay
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14
Southern, Northern blotting
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15
Western Blotting
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16
Biosensors, Transducers
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Total Lessons
16
Department
Biotechnology
About
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Teacher
Dr. Arif Muhammad Khan
Other Courses by
Dr. Arif Muhammad Khan
Research Methods in Biotechnology
Introduction to Biotechnology
Recombinant DNA Technology