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Methods in Molecular Biology
Western Blotting
Western Blotting
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14-western.pdf (2.71 MB )
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Course Material
Solution dilutions
Sterilization techniques
DNA/RNA extraction techniques
Analysis of nucleic acids by gel electrophoresis Horizontal, vertical, pulse field, denaturing gradient gel electrophoresis
Analysis of proteins by native and SDS-PAGE; 2-D gels
Polymerase chain reaction (PCR)
Types of PCR (inverse, touch-down, nested, hemi-nested, pit stop
Multiplex, reverse transcriptase, RACE
Real-time qPCR
Applications of PCR
Detection of mutations and/or SNPs
Detection of mutations and/or SNPs
Enzyme-linked immunosorbant assay
Southern, Northern blotting
Western Blotting
Biosensors, Transducers
Chapters
16
Department
Biotechnology
Teacher
Dr. Arif Muhammad Khan