Types of PCR (inverse, touch-down, nested, hemi-nested, pit stop
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Course Material
- Solution dilutions
- Sterilization techniques
- DNA/RNA extraction techniques
- Analysis of nucleic acids by gel electrophoresis Horizontal, vertical, pulse field, denaturing gradient gel electrophoresis
- Analysis of proteins by native and SDS-PAGE; 2-D gels
- Polymerase chain reaction (PCR)
- Types of PCR (inverse, touch-down, nested, hemi-nested, pit stop
- Multiplex, reverse transcriptase, RACE
- Real-time qPCR
- Applications of PCR
- Detection of mutations and/or SNPs
- Detection of mutations and/or SNPs
- Enzyme-linked immunosorbant assay
- Southern, Northern blotting
- Western Blotting
- Biosensors, Transducers
- Chapters 16
- Department Biotechnology
- Teacher
Dr. Arif Muhammad Khan
