Course Code: CHEM-413
Credit Hours: 3
Instructor name: Dr Yasmeen Gull
Email. [email protected]
DESCRIPTION& OBJECTIVES
This course is designed to provide an understanding about the modern seperation techniques. It will provide a useful knowledge of Gas Chromatography. A detailed survey of HPLC over conventional seperation techniques would also be discussed. After studying this course, students will be able to understand the details of all basic aspects of these techniques.
LEARNING OUTCOMES
The students will acquire knowledge about principles, instrumentation and applications of important chromatographic techniques such as GLC, HPLC. After acquiring the technical knowledge of this course, students will be able to apply these chromatographic techniques on practical grounds for qualitative and quantitative analysis of different chemical samples and characterization of various industrial and natural materials.
COURSE CONTENTS:
Gas–Liquid chromatography, concepts of theoretical plates, Van – deemter equation, High– performance liquid chromatography, instrumentation and applications of these techniques..
Recommended Books
1. Christian, G.D. Analytical Chemistry, John Wiley & Sons, NY (2005).
2. Harris, D.C. Quantitative Chemical Analysis, Freeman, NY (2003).
3. Skoog, D.A. West, D. Holler, J. M.F., Crouch S. R. Fundamentals of Analytical
Chemistry. 8th ed. Saunders College Publishing, Philadelphia (2003)
|
Week |
Topics and Readings |
Dates |
|
1 |
Introduction and basics of chromatography ( Reference Books GD book) |
October 12-16, 2020 |
|
2 |
Historical developments in chromatography and classification (, Reference Books) |
October 19-23, 2020 |
|
3 |
Classification of chromatography (brief overview of adsorption, partition, size exclusion, ion exchange and affinity chromatography (s, Reference Books) |
October 26-30, 2020 |
|
4 |
Introduction/ principle and classification of gas chromatography (GC) , Reference Books) |
November 02-06, 2020 |
|
5 |
Overview of instrumentation of gas chromatography (, Reference Books) |
November 09-13, 2020 |
|
6 |
Details about GC instrument ( Sample injection, gas supply, and columns) (PPT Lectures, Reference Books) |
November 16-20, 2020 |
|
7 |
Details about GC instrument ( Detectors, Integrators and data handling ) (s, Reference Books) |
November 23-27, 2020 |
|
8 |
Principle and instrumentation of GC-MS ( Reference Books) |
November 30 - December 04, 2020 |
|
9 |
Applications of GC and GC-MS in various industrial fields and research & developments Reference Books) |
December 7-11, 2020 |
|
10 |
Mid Term Exams |
December 14-18, 2020 |
|
11 |
Winter Break |
December 21-24, 2020 |
|
12 |
Concepts of theoretical plates, Van – deemter equation |
December 28-31, 2020 |
|
13` |
Introduction to liquid chromatography and high performance liquid chromatography (HPLC) Reference Books) |
January 3-7, 2021 |
|
14 |
Theory, principle and overview of instrumentation of HPLC (s, Reference Books) |
January 10-13, 2021 |
|
15 |
Sample preparation and pre-analysis chemistry (PPT Lectures, Reference Books) |
January 16-20, 2021 |
|
16 |
Instrumentation of HPLC; details of sample injection, solvent supply and pumps systems, isocratic and gradient elution etc. (PPT Lectures, Reference Books) |
January 23-27,2021 |
|
17 |
Instrumentation of HPLC; details of columns, detectors and integrators (PPT Lectures, Reference Books) |
January 30- Feb. 03, 2021 |
|
18 |
Applications of HPLC and LC-MS (PPT Lectures, Reference Books) |
Feb. 05-09, 2021 |
|
19 |
Final Term Exam |
Feb. 08-12, 2021 |
RESEARCH PROJECT/ASSIGNMENT:
The students will be given chromatography related assignments and they will prepare slides for presentation for training purposes.
ASSESSMENT CRITERIA:
Mid Term Examination: 30%
Sessional: 20%
Presentation: Included in sessional marks
Final Examination: 50%
RULES AND REGULATIONSES AND REGULATIONS
- Minimum attendance 75% is necessary to appear in exam.
- mobiles to be off/silent during class time and no permission of using mobile in exam
- as per university academic policy
Course Material
- Total Lessons 2
- Department Chemistry(SCB)
- About Visit Profile
-
Teacher
Dr.Yasmeen Gull
