Credit Hours:        (3+1)

Instructor: Dr Yasmeen Gull

Email. [email protected]

DESCRIPTION& OBJECTIVES

 This course is designed about the Atomic Absorption spectrophotometer and Atomic Emission spectrometer detail. UV/Visible and atomic spectroscopy  is another major topic in this course.This course is developed to enhance the  skills in the student to understand the instrumentation of UV/Visible spectrophotometer.

LEARNING OUTCOMES

The students will able to understand

1. Introduction of Spectroscopy, Classification, Basic terminology

2. AAS instrumentation

3. AAS applications

4. UV/Visible  spectroscopy 

READINGS

1. Skoog D. and West D.M., Fundamentals of Analytical Chemistry, Holt Reinhart Inc, London. (1996).
2. Christian G.D., Analytical Chemistry, John Wiley & Sons. (2010).
3. Harris D.C., Quantitative Chemical Analysis, Freeman, N. Y. (2006).
4. Douglas A. Skoog, Donald M. West, F. James Holler, Stanley R. Crouch Fundamentals of Analytical Chemistry; 8th ed. Saunders College Publishing, Philadelphia. (2003).
5. C. W. Robinson, Undergraduate instrumental analysis, 6th Edition.

CONTENTS

Atomic Spectrometry: Atomic Absorption and Flame Emission Spectrometry, instrumentation and applications, Emission Spectrometry with plasma and electrical discharge sources, UV/Visible Spectrophotometry: basic principle, instrumentation and applications.

RESEARCH PROJECT AND PRACTICALS

Presentation on different Research Topics as a part of assignment

Analytical Chemistry Lab. (Cr.01)

1. Measurement of λ-max and calculation of Molar absorptivity of potassium permanganate.

2. Plotting of calibration graph and measurement of unknown sample concentration.

3. Use of standard addition method in Spectrophotometry.

4. Determination of iron (II) using 1, 10-phenanthroline method.

5. Determination of iron (III) using thiocyanate method involving solvent extraction.

6. Determination of phosphate by Spectrophotometry using molybdenum blue method.

7. Determination of Sodium in tap water sample by using Flame photometer.

8. Determination of Potassium in tap water sample by using Flame photometer.

9. Determination of Calcium in chalk sample by using Flame photometer.

10. Determination of Calcium in drinking water by EDTA.

11. Identification of free salicylic acid in aspirin by using TLC.

12. Determination of Methylene blue value of activated charcoal. 

13. Determination of iron in tap water by AAS.

14. Determination of copper content in milk samples by AAS.

SSESSMENT CRITERIAITERIA

Mid Term Exam: 30%

Sessional:  20%

Presentation:  Included in sessional marks

Participation:  Included in sessional marks

Final exam: 50%

RULES AND REGULATIONS

1. Minimum attendance 75% is necessary to appear in exam.