CELL AND TISSUE CULTURE BIOT-406

Course Code: BIOT-406

Credit Hours: 3(2+1)

Instructor:  Ms. Fareeha Nosheen

Email: [email protected]

DESCRIPTION

The aim of this course is to provide a basic knowledge to students with a thorough understanding of the importance of cell, tissue and organ culture, cell ines and its application in life sciences.

LEARNING OUTCOMES

To acquaint students with techniques and skills of tissue culturing both in animals and plants,. 

CONTENTS

  1. Plant cell and tissue culture:
  2. Requirements for in vitro cultures;
  3. Culture facilities;
  4. Sterile techniques;
  5. Media preparation and handling;
  6. Callus cultures;
  7. Cell suspension cultures;
  8. Protoplast culture;
  9. Haploid cultures,
  10. Organ culture;
  11. Meristem culture for virus elimination;
  12. Embryo culture and embryo rescue;
  13. Regeneration of plants and micropropagation;
  14. Somaclonal variation;
  15. Industrial uses of plant cell culture;
  16. Tissue culture in genetic engineering and biotechnology.
  17. Mammalian cell culture:
  18. Origin and principles of cell culture;
  19. Qualitative characteristics of cell cultures;
  20. Cell counting and analysis;
  21. Cryopreservation;
  22. Cell banking and subculture (variety of different systems);
  23. Primary cell culture techniques;
  24. Development of immortalized cell line;
  25. Detection of microbial contaminants;
  26. Animal cells for bioassays and bioproducts;
  27. Growth environment;
  28. Stem cell culture;

READINGS

  1. Setlow, J.K. 2000. Genetic Engineering: Principles and Methods. Kluwer Academic Publishers.
  2. Nicholl, D.S.T. 2002. An Introduction to Genetic Engineering. 2nd Edition. Cambridge University Press.
  3. Gale, Y.L. 2002. Genetic Engineering.
  4. Razdan, M.K. 2003. Introduction to Plant Tissue Culture. 2nd Edition. Intercept, New York, USA.
  5. Lanza, et al., 2000. Principles of Tissue Engineering. 2nd  Edition. Academic Press, California.
  6. Ignacimutu, S. 1997. Plant Biotechnology. Oxford IBH Publisher.
  7. Punia, M.S. 1999. Plant Biotechnology and Molecular Biology: A Laboratory Manual. Scientific Publishers.

COURSE SCHEDULE

Week

Topics and Readings

Books with Page No.

 

1

Plant cell and tissue culture: Requirements for in vitro cultures; Culture facilities; 

 

2

Sterile techniques; Media preparation and handling;  

 

3

Callus cultures; Haploid cultures, 

 

4

Cell suspension cultures; Protoplast culture;

 

5

Organ culture; Meristem culture for virus elimination;

 

6

Regeneration of plants and micropropagation;

 

7

Embryo culture and embryo rescue; Industrial uses of plant cell culture

 

8

Somaclonal variation;

 

9

Mammalian cell culture: Origin and principles of cell culture;

 

10

Qualitative characteristics of cell cultures;

Cell counting and analysis;

 

11

 Cryopreservation; Cell banking;

 

12

Primary cell culture techniques; Development of immortalized cell line;

 

13

Detection of microbial contaminants; Growth environment;

 

14

Animal cells for bioassays and bioproducts; 

 

15

Stem cell culture;

 

 

RESEARCH PROJECT /PRACTICALS /LABS /ASSIGNMENTS

Preparation of different medium and stocks of macronutrients, micronutrients, and hormones; selection of ex-plant, medium preparation and callus induction; culturing Agrobacterium and using it to infect plant callus; selection of transformants; regeneration of plantlets and acclimatization; working on different cell lines; cryopreservation of cell lines: formation of plouripotent stem cells; observation of three germ layers by using chick embryo;

ASSESSMENT CRITERIA

  1. Attendance                                     05
  2. Behavior                                         05
  3. Assignment & presentation            05
  4. Midterm exam                                15
  5. Final exam                                     45
  6. Practical exam                               25

Course Material

Other Courses by Ms. Fareeha Nosheen