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• Plant cell and tissue culture: Requirements for in vitro cultures; Culture facilities;
• Sterile techniques; Media preparation and handling;
• Callus cultures; Haploid cultures,
• Cell suspension cultures; Protoplast culture;
• Organ culture; Meristem culture for virus elimination;
• Regeneration of plants and micropropagation;
• Embryo culture and embryo rescue; Industrial uses of plant cell culture
• Somaclonal variation;
• Mammalian cell culture: Origin and principles of cell culture;
• Qualitative characteristics of cell cultures; Cell counting and analysis;
• Cryopreservation; Cell banking;
• Primary cell culture techniques; Development of immortalized cell line;
• Detection of microbial contaminants; Growth environment;
• Animal cells for bioassays and bioproducts;
• Stem cell culture;