Primary cell culture techniques; Development of immortalized cell line;
In this week, We are discussed about:
- Primary cell culturetechniques
- Development of Immortalization Cell lines
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Course Material
- Plant cell and tissue culture: Requirements for in vitro cultures; Culture facilities;
- Sterile techniques; Media preparation and handling;
- Callus cultures; Haploid cultures,
- Cell suspension cultures; Protoplast culture;
- Organ culture; Meristem culture for virus elimination;
- Regeneration of plants and micropropagation;
- Embryo culture and embryo rescue; Industrial uses of plant cell culture
- Somaclonal variation;
- Mammalian cell culture: Origin and principles of cell culture;
- Qualitative characteristics of cell cultures; Cell counting and analysis;
- Cryopreservation; Cell banking;
- Primary cell culture techniques; Development of immortalized cell line;
- Detection of microbial contaminants; Growth environment;
- Animal cells for bioassays and bioproducts;
- Stem cell culture;
- Chapters 15
- Department Biotechnology
- Teacher
Ms. Fareeha Nosheen
