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• Introduction and History of Recombinant DNA technology
• vectors and their types
• cloning vectors including plasmids, bacteriophages, cosmids, YAC vectors, shuttle vectors
• DNA modifying enzymes, Restriction Endonucleases
• Restriction Mapping
• Transformations, Cloning strategies
• Expression Vectors
• genomic and cDNA libraries, chromosome walking; Sequencing strategies
• Tumor inducing (Ti) plasmids;
• Expression in prokaryotes and eukaryotes
• Site-directed mutagenesis
• Expression of recombinant proteins
• Expression of recombinant proteins
• Proteins and their purification by affinity chromatography
• Application of recombinant DNA Technology in agriculture
• Application of recombinant DNA Technology in environment and basic research
• Application of recombinant DNA Technology in health industry