genomic and cDNA libraries, chromosome walking; Sequencing strategies
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Course Material
- Introduction and History of Recombinant DNA technology
- vectors and their types
- cloning vectors including plasmids, bacteriophages, cosmids, YAC vectors, shuttle vectors
- DNA modifying enzymes, Restriction Endonucleases
- Restriction Mapping
- Transformations, Cloning strategies
- Expression Vectors
- genomic and cDNA libraries, chromosome walking; Sequencing strategies
- Tumor inducing (Ti) plasmids;
- Expression in prokaryotes and eukaryotes
- Site-directed mutagenesis
- Expression of recombinant proteins
- Expression of recombinant proteins
- Proteins and their purification by affinity chromatography
- Application of recombinant DNA Technology in agriculture
- Application of recombinant DNA Technology in environment and basic research
- Application of recombinant DNA Technology in health industry
- Chapters 17
- Department Biotechnology
- Teacher
Dr. Arif Muhammad Khan
