Week 3: Recombinant DNA methodology, Site directed Mutagenesis, DNA sequencing
Chapter 17: Recombiant DNA Technology, page 338,
In book
Essentials of Genetics, Global Edition by William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino (Ninth Edition) Pearson Education Limited, 2016.
Chapter 7: Creating mutations, page 231,
In book
Analysis of Genes and Genomes by Richard J. Reece. John Wiley & Sons, Limited, 2004
Course Material
- Week 1: Recombinant DNA: Recombinant DNA Technology Introduction, Basic Techniques, PCR and Rt PCR
- Week 2: Restriction enzymes, Plasmids, Bacteriophages as tools, the formation of recombinant DNA
- Week 3: Recombinant DNA methodology, Site directed Mutagenesis, DNA sequencing
- Week 4: Applications of recombinant DNA technology using prokaryotes, recombinant DNA technology in eukaryotes
- Week 5: An overview, transgenic yeast, transgenic plants, transgenic animals
- Week 6: Screening for genetic diseases, identifying disease genes, DNA typing, gene therapy, genetically modified organisms and apprehensions
- Week 7: Mechanisms of Genetic Change I: Gene Mutation: The molecular basis of gene mutations, spontaneous mutations, induced mutations, reversion analysis
- Week 8: Mutagens and carcinogens, Biological repair mechanisms
- Week 9: MID TERM EXAMINATION
- Week 10: Recombination: General homologous recombination, the holiday model, enzymatic mechanism of recombination
- Week 11: Site-specific recombination, recombination and chromosomal rearrangements
- Week 12: Transposable Genetic Elements: Insertion sequences, transposons, rearrangements mediated by transposable elements
- Week 13: Review of transposable elements in prokaryotes, controlling elements in maize
- Week 14: Human Genome Project: Strategies and application, achievement and future prospects
- Week 15: Plant Genome Projects: Arabidopsis, achievement and future prospects. Other plant genome projects
- Week 16: Bioinformatics: Application of computational tests to the analysis of genome and their gene products
- Week 17: Bioethics: Moral, Religious and ethical concerns
- Week 18: FINAL TERM EXAMINATION
- Chapters 18
- Department Botany
- Teacher