Week 16 (Protein purification, characterisation, PCR, SDS PAGE)
- DNA and protein sequencing techniques
- Homogenization methods,
- centrifugation,
- Protein, DNA estimation
- Polymerase chain reaction and its types, applications
- SDS PAGE, applications
Reading List
-
Lehninger, A. L. Principles of Biochemistry, W. H. Freeman; Seventh edition (2017)
-
Voet, D. and J. G. Voet,. Fundamentals of Biochemistry: Life at the Molecular Level, Wiley; 5 edition (2016)
Course Material
- Week 1 (Introduction to Molecular Biology, Eukaryotic and Prokaryotic Replication)
- Week 2 (DNA Damage, Repair and recombination)
- Week 3 (Control of DNA replication, Virus replication, Transcription)
- Week 4 (Operons, Promoters, RNA Polymerases)
- Week 5 (Protein-DNA interaction, Post transcriptional processing)
- Week 6 (Gene expression, rRNA, tRNA)
- Week 7 (Genetic Code, codons and tRNA)
- Week 8 (tRNA activation, Proofreading)
- Week 9 (Translation - Initiation)
- Week 10 (Translation - Elongation complex formation)
- Week 11 (Translation - Termination)
- Week 12 (Control of Protein Synthesis, PTMs)
- Week 13 (Post Translational Modification)
- Week 14 (Protein sorting and degradation)
- Week 15 (Promoters and control of gene expression)
- Week 16 (Protein purification, characterisation, PCR, SDS PAGE)
- Week 17 (Bio-Physical Techniques I)
- Week 18 (Bio-Physical Techniques II)
- Chapters 18
- Department Chemistry
- Teacher
Dr Muhammad Fayyaz ur Rehman
