Week 4: Cosmids and expression vectors, Gene splicing, Chromosome walking, Development of gene libraries
Course Material
- Week 1: Basic molecular Genetic Mechanisms
- Week 2: Structure and Chemistry of nucleic acids, RNA and Proteinsynthesis
- Week 3: Gene Cloning and Sequencing Outline of DNA cloning methods, Cloning vectors including plasmids, Bacteriophages
- Week 4: Cosmids and expression vectors, Gene splicing, Chromosome walking, Development of gene libraries
- Week 5: DNA Amplification and Gel Electrophoresis, Gene sequencing. Potentials of recombinant DNA technology
- Week 6: Introduction to DNA amplification and polymerase chain reaction (PCR). Gel electrophoresis of DNA
- Week 7: Pulse-field agarose gel electrophoresis, Southern blotting, Northern and western blotting, In situ hybridization, DNA microarray.
- Week 8: Transgenic Proteins, Organisms and Gene Therapies
- Week 9: Mid Term Examinations
- Week 10: Reproduction of transgenic proteins, transgenic organisms and gene therapies
- Week 11: Random amplified polymorphic DNA (RAPD)
- Week 12: Microsatellite/SSR, Restriction fragment length polymorphism (RFLP)
- Week 13: Amplified fragment length polymorphism (AFLP).
- Week 14: Molecular definition of a gene, Analysis of gene structure and expression
- Week 15: Deactivating the function of specific genes in eukaryotes, Prokaryotes (E. coli)
- Week 16: Eukaryotes (Arabidopsis thaliana and yeast) and Human genome project.
- Week 17: Genetic and physical mapping of gene
- Week 18: Final Term examinations
- Chapters 18
- Department Botany
- Teacher
Dr. Aamir Ali
