Analytical Chemistry (CHEM-311)

BRIEF INTRODUCTION

Analytical chemistry is concerned with the chemical characterization of matter. It deals with the qualitative as well as quantitative analysis of matter. Many techniques related to separation, purification, estimation etc are studied under the umbrella of this subject.

LEARNING OUTCOMES & OBJECTIVES

Students will be able to learn:

  1. Introduction to analysis and analytical chemistry
  2. Sampling methods
  3. Different methods of analysis
  4. Statistical treatment of data and its processing
  5. Calibration of glasswares and weighing balance
  6. Separation techniques used in analytical chemistry
  7. Thin layer chromatography
  8. Ion-Exchange chromatography and amino acid analyzers

BOOKS:

  1. Skoog, D. A. West, D. Holler, J. M.F., Crouch S. R. Fundamentals of Analytical Chemistry. 8th ed. Saunders College Publishing, Philadelphia. (2003).
  2. Christian, G.D. Analytical Chemistry, John Wiley & Sons, NY (2005).
  3. Harris, D.C.  Quantitative Chemical Analysis, Freeman, NY (2003).


 

CONTENTS

Data Handling: Introduction to Analytical Chemistry, Sampling; Types of samples, Techniques/ Steps involved in sample preparation, Drying and ignition, Weighing, Analytical balance, its construction working volumetric glassware, Errors in measurements, Calibration of glassware, Steps involved in chemical analysis, System for units of measurements and their inter conversion, Chemical concentration and preparation of solutions, Calibration and calibration curves(construction and interpretation), Standard addition and internal standard methods, Statistical treatment of analytical data; Precision, accuracy and types of errors, Sample, Population, Mean, Average, Median, Range, Standard Deviation, Variance, Significant figures, Chemical Equilibrium ant its types.

Separation Techniques: Chromatography (Introduction, Classification.)  TLC. Column & Ion exchange chromatography (with reference to principles & applications). Electrophoresis & Solvent Extraction.

 

COURSE SCHEDULE

 

Sr.#

Topics

Weeks

1.

Introduction to analytical Chemistry and types of analysis

Week 1

2.

Types of samples, Sampling and Sample Storage

Week 2

3.

Steps involved in sample Preparation

Week 3

4.

Methods of Analysis

Week 4

5.

Modern methods of analysis

Week 5

6.

Figures of merits of a method

Week 6

7.

Significant Figures and reporting of results

Week 7

8.

Statistical treatment of data

Week 8

9.

Errors and its types

Week 9

10.

Calibration and methods of calibration

Week 10

11.

Calibration of glassware and analytical balance

Week 11

12.

Chemical equilibrium and its types

Week 12

13.

Concentration and its units, solvent extraction

Week 13

14.

Thin Layer Chromatography and Ion exchange Chromatography

Week 14

15.

Amino Acid analyzer

Week 15

16

Final term

Week 16
 

Analytical Chemistry Lab. (Cr.01)

  1. 1. Calibration of glass ware (Pipette, Burette, Flask) used for volumetric Analysis.
  2. Use of Analytical balance and calculation of standard deviation.
  3. Use of pH meter for plotting acid - base titration curve and assay of commercial caustic soda.
  4. Plotting of first differential curve for titration of acetic acid and commercial soda.
  5. Measurement of solubility products of sparingly soluble salts.
  6. Determination of HCl by titrating with NaOH and plotting of a titration curve.
  7. Packing of chromatographic column and separation of mixture of dyes.
  8. Separation of various components of plant extract by column chromatography.
  9. Separation of mixture of dyes by Radial chromatography.
  10. Separation of mixture of Amino acids by paper chromatography.
  11. Coating of TLC plates and separation of mixture of dyes.
  12. Separation of mixture of Amino acids by TLC.

ASSESSMENT CRITERIA

Sessional: 15% (It includes assignments, presentation, class attendance and class participation)

Mid exam: 15%

Final exam: 45%

Practical assessment: 25%

 

Course Material

Other Courses by Mr. Abu Bakar Siddique