Introduction, syllabus, lecture schedule recommended books

UNIVERSITY OF SARGODHA

DEPARTMENT OF PLANT BREEDING & GENETICS

 

 

COURSE OUTLINE                                                                                                                                           SPRING 2020

 

Course Title:  Molecular Genetics

Course Code:  PBG-310

Credit Hours:        3(2-1)

Instructor: Dr. Saeed Rauf

Email:    [email protected]

DESCRIPTION & OBJECTIVES

 

  • to build understanding of the structure, function, expression and replication of genetic material
  • regulation of gene expression
  • to familiarize student with basic concept of
    • recombinant DNA technology
    • development of transgenic organisms
    • marker assisted selection
    • Integration of novel molecular techniques with plant breeding

 

READINGS

 

Text Books

  1. Klug, W. S.  and M. R. Cummings. 2010. Concepts of Genetics. Dorling Kindersley, Pvt.(Ltd.) New Delhi, India.
  2. Brown T.A. 2012. Introduction to Genetics: A Molecular Approach. 2nd ed., Garland Science, Taylor & Francis, New York and London.
  3. Bilgrami, K. S. and A. K. Pandey. 1992. Introduction of Biotechnology. CBS Publishers & Distributers, New Delhi, India.
  4. Maniatis, T., E. F. Fritsch and J. Sambrook, 1989. Molecular Cloning. A Laboratory Manual. Cold Spring Harbour, USA.

 

 

Reference Material

Beurton PJ, Raphael Falk, Reheinberge HJ (2002): The concept of gene in development and evolution historical and epistemological perspective. Cambridge University Press.

 

 

CONTENTS

  • DNA as genetic material, chemistry of nucleic acids
  • DNA replication; types of RNA, DNA transcription and translation.
  • Transposable elements, Construction of genetic linkages map.
  • Recombinant DNA technique, Genetic transformation, various types and techniques of developing transgenic plants.
  • Marker assisted analysis and QTL mapping.
  • Features of the genetic code, split gene and redundant DNA.
  • Gene mutation, molecular basis of gene mutation, factors affecting mutation rate.

 

 

 

COURSE SCHEDULE

                         

Week

Lecture

Course Schedule

 

Date

Topic

Source

Assignment

1

 
  1.  

The origin of molecular genetics

 

Book 2: 1-10

 

Assignment1: Presentation of 3D model of DNA

13-1-2020 to 17-1-2020

Book 2; Chapter 2: 12-23

 
  1.  

Heredity material Protein vs. Nucleic acid

 

Book 1:2-18

Assignment No2: Cell Structure and Functions

2

 
  1.  

Nucleic acid:

Structure of DNA

Book 1:Chapter 2:21-47

 

20-1-2020 to 24-2-2020
  1.  

Genetic Codon and Structure of DNA

Book 2; Chapter 7: 113-129

 

3

 

 
  1.  

Replication of DNA molecule

Book 1:Chapter 3:53-79

Book 2: Chapter 10 186-205

 

 

 

 

27-1-2020 to 31-1-2020
  1.  

Enzymatic machinery for replication

Book 1:Chapter 3:53-79

Book 2: Chapter 11: 153-168

Internet material

 

4.
  1.  

Genes, prokaryotic genes

Genes in eukaryotic nucleus

Book 2: Chapter 31-48

 

3-2-2020 to 7-2-2020
  1.  

Gene Expression:

Transcription

 

Book 2: Chapter 4; 49-58

 

5.
  1.  

Types of RNA molecules

Book 2: Chapter 5; 75-94

 

10-2-2020 to 14-2-2020
  1.  

Translation;

Information transfer and Protein synthesis

Book 2: Chapter 5; 95-111

Chapter 8;

133-155

 

6.
  1.  

Transposable elements

Book 2: Chapter 6; 52-66

 

17-2-2020 to 21-2-2020
  1.  

Insertion sequences (IS); Composite Transposons; Excision of transposable elements

Book 2: Chapter 7; 86-96

Determination of genetic diversity through molecular markers (Collection of Reference material)   

7.
  1.  

Rearrangement in host DNA

Transposable elements of Eukaryotes

Book 5; Chapter 11: 153-168

 

2-3-2020 to 6-3-2020
  1.  

Mid Term Examination

8.

 
  1.  

Application  of transgenic Plant

Book4: Chapter8: 331-391.

Assignment 4: Genetically engineered diseases resistance in crop specie

9-3-2020 to 13-3-2020
  1.  

Invitro Amplification of DNA by the polymerase

Book 6: Chapter 2 Page 179.

 

9.

 

 
  1.  

Plant Biotechnology and its application

Book4: Chapter8: 283-330.

 

16-3-2020 to 20-3-2020
  1.  

Plant Biotechnology and its application

Book4: Chapter8: 283-330.

Assignment 5: Role of Plant breeding in post genomic era

10.
  1.  

Class Discussion and Quiz

23-3-2020 to 27-3-2020
  1.  

Development of molecular marker for use in plant genotyping

Book 3: 179-225

 

11.
  1.  

Development of molecular marker for use in plant genotyping

Book 3: 179-225

 

12.
  1.  

Recombinant DNA technique,

Book2: Chapter8: 331-391.

 

30-3-2020 to 3-4-2020
  1.  

Genetic transformation various types

Book4: Chapter8: 331-391.

 

13
  1.  

Development of transgenic plants

 

 

6-4-2020 to 10-4-2020
  1.  

Mutation and DNA Repair 

The causes of mutations 

Errors in replication are a source of point mutations  Replication errors can also lead to insertion and deletion mutations

Book 2:

Chapter: 16

313-317

 

14
  1.  

Mutagens are one type of environmental agent that causes damage to cells There are many types of chemical mutagens 

There are also several types of physical mutagens

Book 2:

Chapter: 16

318-322

 

13-4-2020 to 17-4-2020
  1.  

16.2 DNA repair  Direct repair systems fill in nicks and correct some types of nucleotide modification Many types of damaged nucleotide can be repaired by base excision Nucleotide excision repair is used to correct more extensive types of damage Mismatch repair corrects errors of replication 

 

Book 2:

Chapter: 16

323-330

 
  1.  

DNA breaks can also be repaired In an emergency, DNA damage can be bypassed during genome replication

Defects in DNA repair underlie human diseases, including cancers

Book 2:

Chapter: 16

331-336

 

20-4-2020 to 24-4-2020

15
  1.  

Control of Gene Expression The importance of gene regulation

Gene regulation enables bacteria to respond to changes in their environment

Gene regulation in eukaryotes must be responsive to more sophisticated demands 

 

Book 2:

Chapter: 9

157-160

 

27-4-2020 to 1-5-2020
  1.  

Four genes are involved in lactose utilization by E. coli 162 The regulatory gene codes for a repressor protein 164 Glucose also regulates the lactose operon. Operons are common features in prokaryotic genomes

Book 2:

Chapter: 9

161-169

 

16.
  1.  

Final Class Discussion/ Final Examination

 
                     

 

RESEARCH PROJECT

 

  1. 3-D DNA model

 

ASSESSMENT CRITERIA

 

Sessional:                              8 (Class Attendance: 2, Presentation: 4, Assignments: 2)

Mid Term Test:    12

Final Term Test:  20

RULES AND REGULATIONS

 

1.  Bonus marks according to student contributions in class/lecture discussions

2: Students have to complete all assignments within Targets