ZOL-503 -Cell and Molecular Biology (BS-Zoology), ZOOL-6203 (MSc-Zoology

Couse Title:               Cell and Molecular Biology

Course Code:            ZOL-503

Cr. Hrs:                      3+1

Classes:                   BS 5th Semester (Regular  & Self Support)

                                 MSc 1st Semester (Regular)

Course Instructor:    Dr. Shafaat Yar Khan

 

Pre-requisite: Undergrad level understanding of Cell and Molecular Biology

Learning outcomes:

The course will impart knowledge about the animal cell and its complex organization of architecture and will provide understanding about the unified role of a cell for the ultimate sustainability of the organisms. The contents will enable students to understand various ultra-structural, molecular and functional aspects of the cells will be communicated in this course

Other requirements:

  1. A functional email address
  2. An internet connection
  3. Good use of MS office program
    1. MS Word,
    2. MS power point,
    3. Adobe Acrobat reader (for pdf.)

The students who will enroll in the course will get relevant reading material at the start of the course.

Course Contents:

  1. Introduction to prokaryotic and eukaryotic cells
  2. Cytoskeleton
  3. Cytoplasmic Organelles: Endoplasmic Reticulum, Golgi apparatus, Mitochondria and Mitochondrial respiration; Lysosome, Peroxisome, Glycoxysome .
  4. Nucleus
  5. DNA Replication 
  6. Transcription
  7. Posttranscriptional processing
  8. Genetic code, point mutations.
  9. Translation

Readings:

  • Alberts, B., Bray, D., Lewis, J., Raff, M., Roberts, K., Watson, J.D. Molecular Biology of the Cell. (6th edition Garland Publishing Inc. New York, 2017)
  • De Robertis, E. D. P. Cell and Molecular Biology (8th Edition, Lea & Febiger, New York, 2017)
  • Karp, J. Cell and Molecular Biology, Concepts and Experiments (Jhon Wiley and Sons, INC, 2005.)
  • Damell Jr. J., Lodisch, H., Balimore, D. Molecular Cell Biology. (Scientific American Inc.New York, 2013)
  • Geoffrey M.C., Robert E.H. The cell: A Molecular Approach (Sinauer Associates, INC, 2007).
  •  Online sources

  1. Practicals

  2. Identification of cell organelles
  3. Preparation of temporary whole mount.
  4. Preparation of permanent whole mount (demonstration)
  5. Preparation of human blood smear and identification of Leucocytes.
  6. Tissues (permanent slides of epithelial tissues, striated muscle, smooth muscle, cartilage, bone).
  7. Squash preparation of onion root tip for mitotic stages.
  8. Mounting of polytene chromosome (Drosophila/Chironomous.) Demonstration.
  9. Detection and quantitative determination of chromosomal DNA and RNA.
  10. Cultural and staining of bacteria and yeast.
  11. Separation of different sized DNA fragments on agarose gel.
  12. Isolation and characterization of proteins on polyacrylamide gel electrophoresis (native and sub-unit molecular weights).

ASSESSMENT CRITERIA

Sessional: 10% (Presentation:  5% and Participation:  5%)

Practical: 15%

Midterm exam/ Term paper: 25 %

Final exam: 50%

  1. Time Table:                    

    BS 5th Semester Regular (2018-2022) Spring semester

    Tuesday

    08:00-10:00 AM

    Tuesday

    11:00-12:00 PM

    Wednesday

    12:00-1:00 PM

    Thursday

    9:00-10:00 AM

     

    BS 5th Semester Self Support (2018-2022) Spring semester

    Monday

    03:00-4:00 PM

    Tuesday

    03:00-4:00 PM

    Wednesday

    03:00- 4:00 PM

    Thursday

    02:00- 4:00 PM

     

    M.Sc. 1st Semester Regular (2020-2022) Spring semester

    Tuesday

    01:00-02:00 PM

    Wednesday

    01:00-02:00 PM

    Friday

    09:00- 10:00 AM
    .

Download Files

karp.pdf (31.75 MB )