CHEM-614 Advance Spectroscopy-I

COURSE OUTLINE FALL 2020

Course Tittle: ADVANCED SPECTROSCOPY – I (MINOR)

Course Code: CHEM-614

Credit Hours: (3+1)

Instructor: MUHAMMAD SHER

DESCRIPTION& OBJECTIVES

Advance spectroscopic techniques including in this course are very important regarding the identification and characterization of newly synthesized or isolated chemical compounds. These courses develop the skill in the student to understand the instrumentation of UV/Visible spectrophotometer, Atomic Absorption spectrophotometer and Atomic Emission spectrometer. It also develops the skill in the student to interpret the UV/Visible spectra of various compounds for structure determinations. Atomic Absorption and Emission spectroscopy is another major topic in this course, which in turn enable the students to identify and quantify the various metals present in samples. UV/Visible and atomic spectroscopy has number of applications in the various fields of research.

READINGS

Skoog D. and West D.M., Fundamentals of Analytical Chemistry, Holt Reinhart Inc, London. (1996).
Christian G.D., Analytical Chemistry, John Wiley & Sons. (2010).
Harris D.C., Quantitative Chemical Analysis, Freeman, N. Y. (2006).
Douglas A. Skoog, Donald M. West, F. James Holler, Stanley R. Crouch Fundamentals of Analytical Chemistry; 8th ed. Saunders College Publishing, Philadelphia. (2003).
C. W. Robinson, Undergraduate instrumental analysis, 6th Edition.

CONTENTS

Atomic Spectrometry: Atomic Absorption and Flame Emission Spectrometry, instrumentation and applications, Emission Spectrometry with plasma and electrical discharge sources, UV/Visible Spectrophotometry: basic principle, instrumentation and applications.

COURSE SCHEDULE

Week	Topics and Readings	Dates
1.	Introduction to spectroscopy (Molecular and Atomic spectroscopy) Measurement of λmax and calculation of Molar absorptivity of potassium permagnate.(Practical)	12,15,16 October
2.	UV/Visible Spectroscopy: Introduction and basic principle	19, 22, 23 October
3.	Lambert – Beer Law, Derivation of Law and limitation of Beer’s law Plotting of calibration graph and measurement of unknown sample concentration.(Practical	27, 29, 30 October
4.	Instrumentation of UV/Visible Spectrophotometer: Source of Radiation, Monochromator, Sample Holder, Detectors and read out system Use of standard addition method in Spectrophotometry (Practical)	2, 5,6, November
5.	Determination of phosphate by Spectrophotometry using molybdenum blue method. Double Beam and Single Beam Spectrophotometer, Compensated double beam spectrophotometer, Calibration UV/Visible spectrophotometer, Applications of UV/Visible spectroscopy	9, 12, 13, November
6.	Atomic Emission Spectroscopy: Flame Photometry, Basic principle, Introduction to Atomic Absorption Spectroscopy Determination of iron (III) using thiocyanate method involving solvent extraction.	16,, 19, 20 November
7.	Determination of Potassium in tap water sample by using Flame photometer. Flame photometry: Instrumentation and Applications	23, 26, 27 November
8.	Bathochromic and Hypsochromic shift, Conjugation effect, Solvent effect and effect of Auxochrome Determination of iron (II) using 1,10-phenanthroline method.	3,4 December
9	Interferences in Atomic Absorption: Matrix, Chemical and Ionization, Spectral interferences Determination of Sodium in tap water sample by using Flame photometer.	7, 10, 11 December
10	Mid Term	MID 14 December -18 December
11	Instrumentation of Atomic Absorption Spectroscopy: Sources, Flame and Burner, Nebulization and aspiration of sample, Monochromator and detector Determination of Potassium in tap water sample by using Flame photometer	21, 24 December
12	Graphite Atomic Absorption technique, Indirect Method: Cold Vapour Mercury technique, Hydride generation technique, Applications of AA spectroscopy Determination of Calcium in chalk sample by using Flame photometer.	11,14,15 January
13	Determination of Calcium in drinking water by EDTA. Emission Spectroscopy: Arc/Spark emission spectroscopy, Instrumentation	18,21,22 January
14	Emission Spectroscopy: Arc/Spark emission spectroscopy, Inductive coupled plasma emission spectroscopy: Instrumentation Identification of free salicylic acid in aspirin by using TLC.	25,28,29 January
15	Applications of Arc/Spark Emission spectroscopy, Applications of Inductive coupled Plasma Emission spectroscopy Determination of Methylene blue value of activated charcoal.	1,4,5 February
16	Sample preparation for Atomic Absorption and Flame Photometry and presentations of students	25, 28, 29 January
17	Final-Term Examination 08 February to 12 February2021

RESEARCH PROJECT

Analytical Chemistry Lab. (Cr.01)

Measurement of λmax and calculation of Molar absorptivity of potassium permagnate.
Plotting of calibration graph and measurement of unknown sample concentration.
Use of standard addition method in Spectrophotometry.
Determination of iron (II) using 1,10-phenanthroline method.
Determination of iron (III) using thiocyanate method involving solvent extraction.
Determination of phosphate by Spectrophotometry using molybdenum blue method.
Determination of Sodium in tap water sample by using Flame photometer.
Determination of Potassium in tap water sample by using Flame photometer.
Determination of Calcium in chalk sample by using Flame photometer.
Determination of Calcium in drinking water by EDTA.
Identification of free salicylic acid in aspirin by using TLC.
Determination of Methylene blue value of activated charcoal.
Determination of iron in tap water by AAS.
Determination of copper content in milk samples by AAS.