COURSE OUTLINE                                                                                                                                           FALL 2020

 

Course Tittle:        ADVANCED SPECTROSCOPY – I (MINOR)

Course Code:        CHEM-614

Credit Hours:        (3+1)

 

Instructor: MUHAMMAD SHER

Email: [email protected]

DESCRIPTION& OBJECTIVES

 

Advance spectroscopic techniques including in this course are very important regarding the identification and characterization of newly synthesized or isolated chemical compounds. These courses develop the skill in the student to understand the instrumentation of UV/Visible spectrophotometer, Atomic Absorption spectrophotometer and Atomic Emission spectrometer.  It also develops the skill in the student to interpret the UV/Visible spectra of various compounds for structure determinations. Atomic Absorption and Emission spectroscopy is another major topic in this course, which in turn enable the students to identify and quantify the various metals present in samples. UV/Visible and atomic spectroscopy has number of applications in the various fields of research. 

READINGS

  1. Skoog D. and West D.M., Fundamentals of Analytical Chemistry, Holt Reinhart Inc, London. (1996).
  2. Christian G.D., Analytical Chemistry, John Wiley & Sons. (2010).
  3. Harris D.C., Quantitative Chemical Analysis, Freeman, N. Y. (2006).
  4. Douglas A. Skoog, Donald M. West, F. James Holler, Stanley R. Crouch Fundamentals of Analytical Chemistry; 8th ed. Saunders College Publishing, Philadelphia. (2003).
  5. C. W. Robinson, Undergraduate instrumental analysis, 6th Edition.

CONTENTS

Atomic Spectrometry: Atomic Absorption and Flame Emission Spectrometry, instrumentation and applications, Emission Spectrometry with plasma and electrical discharge sources, UV/Visible Spectrophotometry: basic principle, instrumentation and applications.

 

COURSE SCHEDULE

 

Week

Topics and Readings

Dates

1.

Introduction to spectroscopy (Molecular and Atomic spectroscopy)

Measurement of λmax and calculation of Molar absorptivity of potassium permagnate.(Practical)

 

12,15,16  October

2.

UV/Visible Spectroscopy: Introduction and basic principle

 19, 22, 23 October

 

3.

Lambert – Beer Law, Derivation of Law and limitation of Beer’s law

Plotting of calibration graph and measurement of unknown sample concentration.(Practical

27, 29, 30 October

4.

Instrumentation of UV/Visible Spectrophotometer: Source of Radiation, Monochromator,  Sample Holder, Detectors and read out system

Use of standard addition method in Spectrophotometry (Practical)

 

2, 5,6,  November

5.

Determination of phosphate by Spectrophotometry using molybdenum blue method.

Double Beam and Single Beam Spectrophotometer, Compensated double beam spectrophotometer, Calibration UV/Visible spectrophotometer, Applications of UV/Visible spectroscopy

9, 12, 13, November

 

6.

Atomic Emission Spectroscopy: Flame Photometry, Basic principle, Introduction to Atomic Absorption Spectroscopy

 Determination of iron (III) using thiocyanate method involving solvent extraction.

 16,, 19, 20 November

7.

Determination of Potassium in tap water sample by using Flame photometer.

Flame photometry: Instrumentation and Applications

23, 26, 27 November

 

8.

Bathochromic and Hypsochromic shift, Conjugation effect, Solvent effect and effect of Auxochrome

Determination of iron (II) using 1,10-phenanthroline method.

 

3,4 December

9

Interferences in Atomic Absorption: Matrix, Chemical and Ionization, Spectral interferences

Determination of Sodium in tap water sample by using Flame photometer.

7, 10, 11 December

10

Mid Term

MID 14 December  -18 December

11

Instrumentation of Atomic Absorption Spectroscopy: Sources, Flame and Burner, Nebulization and aspiration of sample, Monochromator and detector Determination of Potassium in tap water sample by using Flame photometer

21, 24 December

 

12

Graphite Atomic Absorption technique, Indirect Method: Cold Vapour Mercury technique, Hydride generation technique, Applications of AA spectroscopy Determination of Calcium in chalk sample by using Flame photometer.

11,14,15 January

13

Determination of Calcium in drinking water by EDTA.

Emission Spectroscopy: Arc/Spark emission spectroscopy, Instrumentation

18,21,22 January

 

14

Emission Spectroscopy: Arc/Spark emission spectroscopy,  Inductive coupled plasma emission spectroscopy: Instrumentation

 Identification of free salicylic acid in aspirin by using TLC.

25,28,29 January

15

Applications of Arc/Spark Emission spectroscopy, Applications of  Inductive coupled Plasma Emission spectroscopy

Determination of Methylene blue value of activated charcoal.

1,4,5 February 

 

16

Sample preparation for Atomic Absorption and  Flame Photometry and presentations of students

25, 28, 29 January

17

Final-Term Examination   08 February to 12 February2021

 

 

 

RESEARCH PROJECT

 

Analytical Chemistry Lab. (Cr.01)

 

  1. Measurement of λmax and calculation of Molar absorptivity of potassium permagnate.
  2. Plotting of calibration graph and measurement of unknown sample concentration.
  3. Use of standard addition method in Spectrophotometry.
  4. Determination of iron (II) using 1,10-phenanthroline method.
  5. Determination of iron (III) using thiocyanate method involving solvent extraction.
  6. Determination of phosphate by Spectrophotometry using molybdenum blue method.
  7. Determination of Sodium in tap water sample by using Flame photometer.
  8. Determination of Potassium in tap water sample by using Flame photometer.
  9. Determination of Calcium in chalk sample by using Flame photometer.
  10. Determination of Calcium in drinking water by EDTA.
  11. Identification of free salicylic acid in aspirin by using TLC.
  12. Determination of Methylene blue value of activated charcoal.
  13. Determination of iron in tap water by AAS.
  14. Determination of copper content in milk samples by AAS.

 

ASSESSMENT CRITERIA

 

Mid Term Exam: 15%

Sessional:     15%

Project/Practical work:       25%

Presentation:  included in sessional marks

Participation:  Nil

Final exam: 45%

 

RULES AND REGULATIONS

 

  1. Minimum attendance 75% is necessary to appear in exam.
  2. For practical exam also 75% attendance in practical is necessary.
  3. Keep your mobiles of/silent during class time.

 

Course Material