CHEM-415 ADVANCED SPECTROSCOPY – II (MINOR)

Course Tittle: ADVANCED SPECTROSCOPY – II (MINOR)

Course Code: CHEM-415

Credit Hours: (3+1)

Instructor: MUHAMMAD SHER

DESCRIPTION& OBJECTIVES

Advance spectroscopic techniques including in this course are very important regarding the identification and characterization of newly synthesized or isolated chemical compounds. These courses develop the skill in the student to interpret the mass spectra of various compounds for structure determinations after studying mass spectrometry. Molecular luminescence spectroscopy is another major topic in this course, which in turn enable the students to quantify the various substances. Fluorescence studies are now a day’s play a vital role in the monitoring of drugs at nano level in cancer patients. X-rays spectroscopy is another important field of analysis. This course has number of applications in diagnostic.

READINGS

Skoog D. and West D.M., Fundamentals of Analytical Chemistry, Holt Reinhart Inc, London. (1996).
Christian G.D., Analytical Chemistry, John Wiley & Sons. (2010).
Harris D.C., Quantitative Chemical Analysis, Freeman, N. Y. (2006).
Douglas A. Skoog, Donald M. West, F. James Holler, Stanley R. Crouch Fundamentals of Analytical Chemistry; 8th ed. Saunders College Publishing, Philadelphia. (2003).

CONTENTS

Mass Spectrometry: Principle of Mass spectroscopy, Instrumentation in details, Quantitative and Qualitative application in analytical chemistry. X-rays Spectroscopy: Nature and production of X-rays, X-rays absorption, X-rays emission, Instrumentation, X-rays fluorescence analysis, Diffraction studies single crystal analysis

COURSE SCHEDULE

Week	Topics and Readings	Dates
1.	Mass Spectrometry, Principle of MS, Molecular ion, Base Peak	1st Weak
2.	Resolving power and resolution of a Mass Spectrometry, Instrumentation, Sample Inlet System	2nd Weak
3.	Ionization Sources, Electron Impact source, Chemical Ionization, Atmospheric pressure ionization sources, Laser Desorption, MALDI,	3rd Weak
4.	FAB, Ionization Sources for Inorganic MS	4th Weak
5.	Mass Analyzer, Magnetic Sector Analyzer, Time of flight Analyzer,	5th Weak
6.	Quadrupole Mass Analyzer, Quadrupole Ion Trap,	6th Weak
7.	Detectors, Electron Multiplier, Faraday cup, Array detector	7th Weak
8.	Mid Term Exam	8th Weak
9.	Quantitative and Qualitative applications in Analytical Chemistry, Interpretation of mass spectra: Structural determination of simple molecules	9th Weak
10.	X-ray spectroscopy: Origin of X-ray spectra, Energy Levels in Atoms,	10th Weak
11.	Moseley’s law, X-Ray Methods, x-ray absorption process,	11th Weak
12.	X-ray fluorescence (XRF) process, The X-ray Diffraction (XRD) process	12th Weak
13.	Instrumentation: X-ray Source, Secondary XRF Sources, Radioisotope sources	13th Weak
14.	Collimators, Filters, WDXRF spectrometers, Detectors, Gas filled detectors, Sample Holders,	14th Weak
15.	EDXRF spectrometers: X-Absorption, X-rays Diffraction, Analytical Application of X-Rays	15th Weak
16.	Final-Term Examination	16th Weak

LEARNING OUTCOMES:

The students will get the basic knowledge of mass spectrometry and x-ray spectroscopy along with their instrumentation and applications in analytical chemistry. These courses develop the skill in the student to interpret the mass spectra of various compounds for structure determinations after studying mass spectrometry. Molecular luminescence spectroscopy is another major topic in this course, which in turn enable the students to quantify the various substances. Fluorescence studies are now a day’s play a vital role in the monitoring of drugs at nano level in cancer patients. X-rays spectroscopy is another important field of analysis. This course has number of applications in diagnostic.

Analytical Chemistry Lab. (Cr.01)

Verification of deviations from Beer-Lambert’s law.
Determination of chloride content in drinking water samples bymercury(II) thiocyanate spectrophotometric method.
Determination of copper in various food samples by diethyldithiocarbamate spectrophotometric method.
Determination of aspirin in pharmaceutical preparation and caffeine in tea and coffee by U.V Visible Spectrophotometry involving extraction.
Analysis of analgesic by HPLC.
Quantitative and qualitative analysis of different fruit juices for vitamin C by HPLC.
Estimation of Sodium and Potassium in biological fluids by flame photometry.
Determination of calcium in milk samples by flame photometry.
Determination of Magnesium in tap water, food, leaves etc by AAS.
Determination of manganese content in tea leaves by AAS.
Determination of sulphate and phosphate in commercial samples by complexometric titrations using EDTA.
Determination of iron in pharmaceutical samples by redox titration.
Determination of Sodium bicarbonate contents in baking Soda powder by conductometric titration with HCl.