Objectives:

The subject aims to provide an advanced understanding of the core principles and topics of Biochemistry and their experimental basis, and to enable the students to acquire a specialised knowledge and understanding of selected aspects by means of series of lectures and lab experiments. Through this course the students would be able to acquire fundamental knowledge of the molecules of life (also knowns as biomolecules) such as nucleic acids, carbohydrates, proteins and fatty acids, as well as their function in the context of a living cell asthey provide the body with energy. The students will also become familiar with the biochemical functions of water and buffer inside the cell. The students will also be able to recognize the different classes of enzymes and coenzymes and their role in the biological processes in the body. The students will also get knowledge about hormones in terms of their structure, function and role in regulating the metabolism. 

Contents

1. Introduction to biochemistry 
2. Water, pH, buffers, and biochemical composition of cells
3. Carbohydrates - structure and classification
4. Proteins - overview with emphasis on their composition and structure
5. Classification and function of proteins
6. Lipids - structure, classification and biological significance
7. Enzymes - properties, nomenclature, classification
8. Factors affecting enzyme activity including inhibitors and potentiators, 
9. Basic kinetics, derivation of Kmand Vmax; coenzymes and vitamins
10. Nucleic acids - structure and function

Practicals

Standard laboratory operating and safety procedures,Preparation of laboratory solutions, buffers and pH determination; qualitative and quantitative tests for carbohydrates, proteins and lipids; enzyme assays and the effect of pH, temperature and other factors on enzyme activity. 

Recommended Text

1. Nelson, D.L., & Cox, M. M. (2012). Lehninger principles of biochemistry,(6thed.). New York: W.H. Freeman.
2. Hames, D. & Hooper, N. (2006). Instant notes biochemistry,(3rded.). USA: Taylor & Francis Group.

Suggested Readings

1. Berg, J., Tymoczko, J., & Stryer, L. (2006). Biochemistry,  (6thed.). New York: W.H. Freeman and Company.
2. Voet, D., & Voet , T.G. (2008). Biochemistry,(4thed.). New York: John Wiley & Sons.
3. Murray, et al.,(2012). Harper’s ilustrated biochemistry,(29thed.). New York: McGraw-Hill Medical Publishers.
4. Ferrier, D.R. (2013). Lippincott’s Biochemistry,(6thed.). USA: Lippincott Williams & Wilkin Publishing Company

Assessment Criteria

Sessional: 15 Marks (Quiz: 05, Presentation: 10)

Practical: 25 Marks

Final exam: 45 Marks

Mids: 15 Marks