This course provides comprehensive introduction on UV Spectroscopy/Separations, Quantitative Infrared Spectroscopy, Flame Atomic Absorption Spectroscopy, Direct Potentiometry, Spectro fluorimetry: Determination of Fluid Volumes by Dye Dilution Techniques, TLC, Drug   Monitoring   Techniques, Column Techniques etc. The objective of this course is to provide an introductory survey of modern analytical instrumentation and techniques in the areas of spectroscopy, chromatography, and electroanalysis. Emphasis will be placed on the physical phenomena governing the operation of each instrument and the general components of each instrument. After completing the course, students should be versed in different chemical methods of analysis and able to identify a suitable instrument for a specific application in the laboratory. The laboratory is designed to provide hands-on experience using chemical instrumentation through a combination of experiments and demonstrations. Students should be able to describe the advantages and disadvantages of different instruments in terms of usability, sensitivity, cost, and other parameters with an emphasis on the complementarity of different techniques.


After completion of this course, the student should be able to:

1. Isolation, purification and characterization of different molecules from any sample.

2. Quality control of various products for industrial applications

3. Production of various compounds at industrial scale

Assesment Criteria

Sessional: 20

Midterm exam: 30

Final exam: 50

Class Scedule

Monday       1;00-2;00 PM

Tuesday        1;00-2;00 PM

Wednesday   1;00-2;00 PM


Book Readings

Rodney Boyer, Biochemistry Labortary: Modern Theory and Techniques, 2nd Edition. 

K.L. Khatak, Techniques and Methods in Biology.

Course Material