To acquaint the students with modern techniques of plant multiplication.
Introduction and importance; Basic terminology, application and constraints of plant tissue
culture; Nutritional components of culture media (nutrients, carbohydrates, vitamins, growth
regulators, amino acids and antibiotics), their types & functions; aseptic techniques; Initiation
and maintenance of cultures; Physical factors for growth; transplanting and acclimatization;
Preparation of synthetic seed; Concepts of plant biotechnology and its role in improvement of
horticultural crops.
Laboratory safety precautions, sanitation, equipment; Calculations (preparation of molar,
percent, normal, ppm etc solutions); Preparation of stock solutions & media, sterilization
techniques, inoculation and culture of explants, Acclimatization & transplanting.
Learning Outcomes:
Students must understand the basic tissue culture techniques for in vitro propagation of
horticultural crops.
Books recommended:
Hartmann, H.T., D.E. Kester and F.T. Davies. 2011. Plant propagation: principles and
practices (8th Ed.). prentice Hall, New Jersey, USA.
Lindsey, K. (Ed). 2007. Plant Tissue Culture Mannual. Springer-Kluwer Academic
Publishers New Delhi, India.
George, E.F., M.A. Hall and G.J. De Klerk. 2008. Plant Propagation by Tissue Culture.
3rd Ed. Springer, Dordrecht, Netherlands.
Razdan, M.K. 2003. Introduction to Plant Tissue Culture. Science Publishers Inc. (ISBN:
Scoggins, H. and M. Bridgen. 2014. Plants from Test Tubes: An Introduction to Micro
propagation (4th Ed.) Tiber Press.
Smith,R. H. 2013. Plant Tissue Culture: Techniques and Experiments. Academic Press,
London, UK.
Trigiano, R. N. and J. G. Denis. 2011. Plant Tissue Culture, Development, and
Biotechnology. CRC press, Boca Raton,Folorida,USA.
Margit, L. and W. Rucker. 2004. Plant Tissue Culture: Plant Tissue Culture 100 years
since Gottlieb Haberlandt. CPL Scientific Publishers, (ISBN: 3211838392).
Chandra, R. and M. Mishra (Eds). 2005. Comprehensive Micropropagation of
Horticultural Crops International Book Distributing Co. (Publishing Division), Lucknow,

Course Material