Analytical Chemistry and Instrumentation (BIOT-301)

Introduction to course

  • Analysis Qualitative and Quantitative
  • Analytical analysis
  • Chromatography and its various types
  • GC-MS and LC-MS
  • Atomic Absorption Spectroscopy
  • Luminescence, flourescent, emission spectroscopy
  • UV-Vis and Infra Red spectroscopy
  • Nuclear Magnetic Resonance
  • Introduction to X-Ray diffraction
  • Methods of and characterization of proteins, nucleic acids including dialysis, ultrafilteration, lyophilisation, ultra centrifuge and aminoacid analyzer.

Course pre-requisites (if any)

  • The prerequisites is the Physical Chemistry (CHEM 181), Inorganic Chemistry (CHEM 161) Organic Chemistry (CHEM 271) in minimum grade “C” or better.

Learning outcomes

  • The course will acquire knowledge about basic concepts of analytical chemistry and understanding of various analytical techniques. Such information will be useful for qualitative and quantitative analysis.

Textbooks to be used for the course


  • Younas, M., Organic Spectroscopy, A. H. Publisher, Lahore. (2006).
  • Solomons, T. W. G., Fundamentals of Organic Chemistry, 12th edition Wiley, New York. (2016)
  • Rehman, A., Experimental Organic Chemistry, The Caravan Book House, Lahore. (2006).

Description of system of Evaluation (Exam, assignments etc)

  • Attendance                         5 (Minimum attendance 80% is necessary to appear in exam)
  • Behaviour                            5
  • Assignment & presentation   5
  • Mid Term Exam                   15%
  • Project/Practical work:         25%
  • Final exam:                         45%

Detailed lesson plans for each lecture (Weekly/Week wise)

Week 1

Introduction to analysis and analytical techniques.

Week 2

Introduction to chromatography, its history, basic principle, concept of stationary phase and mobile phase, Rf value 

Week 3

Introduction to column chromatography, types. Liquid solid and liquid liquid chromatography

Week 4

Paper chromatography and Thin layer chromatography

Week 5

Gas chromatography, GC-MS, LC-MS

Week 6

High performance liquid chromatography HPLC, Its principle and instrumentation. Normal phase and Reverse phase chromatography

Week 7

Gel exclusion chromatography

Week 8

Introduction to spectroscopy, various regions of light and their interaction with matter

Week 9

Mid – Term Examination

Week 10

Ultraviolet and visible spectroscopy (UV-Vis spectroscopy) Basic principle, instrumentation and applications.

Week 11

Infra red spectroscopy (IR spectroscopy) Basic principle, instrumentation and applications

Week 12

 Nuclear magnetic resonance, Basic principle, instrumentation and its application. Effect of radiowaves on rotational transitions.

Week 13

Atomic Absorption spectroscopy (AAS)

Week 14

Inductively coupled plasma mass spectrometry (ICPMS)

Week 15

Introduction to X-Ray diffraction

Week 16

Methods of and characterization of proteins, nucleic acids including dialysis, ultrafilteration, lyophilisation, ultra centrifuge and aminoacid analyzer 

Week 17


Week 18

Final Term Examination

Organic Chemistry Lab. – I (Cr.01)

Key dates and time of class meetings

  • Semester Start : October 12, 2020
    • BS Biotechnology V SS
  • Wednesday         2.00-3.00 pm
  • Thursday            2.00-3.00 pm
  • Friday                 2.00-4.00 pm 

Course Material