Objective:
To introduce students to the basic and applied concepts of plant viruses.
Theory:
Introduction, history and importance, morphology, composition and structure; classification of plant viruses, their replication, transmission and movement; symptomatology; serology and serological methods; ecology and epidemiology; management; study of specific virus diseases in Pakistan.
Practical:
Field visits and study of virus infected plants; methods of virus transmission (mechanical inoculation, grafting, insect vectors); virus detection through biological (indicator hosts and host range) and serological methods (ELISA, Immunodiffusion) and molecular methods (polymerase chain reaction)
Students will be able to understand the basic concept of plant virology. This course will also be helpful for the students in their higher studies.
Books:
Week |
Topics and Readings |
Books with Page No.
|
1 |
What is virus and its economic importance, History of virus and symptomology |
Comparative Plant Virology (2nd Ed.,) (3-10, 24) |
2 |
Classification and Replication of plant viruses
|
(Comparative Plant Virology (2nd Ed., (13-17) |
3 |
Replication of RNA and DNA viruses |
Comparative Plant Virology (2nd Ed.,) (140,146,147,152,156,157) |
4 |
Physico-chemical properties of plant viruses, Virus-like Pathogens
|
Comparative Plant Virology (2nd Ed.)(50-57) Plant Viruses: Unique and Intriguing Pathogens,(73-91) |
5 |
Pathological effect of viroids on host plants, Replication of viroid’s |
Comparative Plant Virology (2nd Ed.,)(44-45, 46-47) |
6 |
Transmission of plant viruses, Mechanical transmission
|
(PPT slides and handouts) |
7 |
Seed transmission
|
(PPT slides and handouts) |
8 |
Mid term |
|
9 |
Insect transmission |
(PPT slides and handouts) |
10 |
Fungal and nematode transmission |
(PPT slides and handouts) |
11 |
Beetle and mite transmission |
(PPT slides and handouts) |
12 |
Diagnosis of Plant Viruses |
(PPT slides and handouts) |
13 |
Viral Diseases |
(PPT slides and handouts) |
14 |
Major viral diseases in Pakistan |
(PPT slides and handouts) |
15 |
Major viral diseases in Pakistan |
(PPT slides and handouts) |
16 |
Final Term |
|
Sessional: 20% (Participation, Presentation, Assignment)
Project: -
Presentation: -
Participation: -
Mid Exam: 30%
Final Exam (including practical): 50%