Objective:

To introduce students to the basic and applied concepts of plant viruses.

Theory:

Introduction, history and importance, morphology, composition and structure; classification of plant viruses, their replication, transmission and movement; symptomatology; serology and serological methods; ecology and epidemiology; management; study of specific virus diseases in Pakistan.

Practical:

Field visits and study of virus infected plants; methods of virus transmission (mechanical inoculation, grafting, insect vectors); virus detection through biological (indicator hosts and host range) and serological methods (ELISA, Immunodiffusion) and molecular methods (polymerase chain reaction)

Students will be able to understand the basic concept of plant virology. This course will also be helpful for the students in their higher studies.

Books:

  1. Ahlawat, Y.S. 2010. Diagnosis of Plant Viruses and Allied Pathogens. Stadium Press (India) Pvt. Ltd.
  2. Bashir, M. and S. Hassan. 1998. Diagnostic Methods for Plant Viruses. Pakistan Agricultural Research Council, Islamabad, Pakistan.
  3. Bos, L. 1999. Plant Viruses: Unique and Intriguing Pathogens: A text book of Plant Virology. Backhuys Publishers.
  4. Compendia of different crops. American Phytopathogical Society, St. Paul, Minnesota, USA.
  5. Foster, G.D. and S.C. Taylor. 1998.  Plant Virology Protocols-From Virus Isolation to Transgenic Resistance. Humana Press, New Jersey.
  6. Hadidi, A., R. K. Khetarpal and H. Koganezawa (Eds.). 1998. Plant Virus Disease Control. APS, USA.
  7. Hull, R. 2009. Comparative Plant Virology. 2nd edition. Academic Press.
  8. Loebenstein, G. and G. Thottappilly (Eds.). 2004. Virus and Virus-like Diseases of Major Crops in Developing Countries. Springer Press.
  9. Matthews. R.E.F. 1991. Plant Virology. 3rd revised edition. Academic Press.
  10. Thresh, M. (Ed.). 2006. Plant Virus Epidemiology. Academic press.
  11. Verma H. N. 2003. Basics of Plant Virology. Oxford and IBH Publishing CO. PVT. LTD

 

Week

Topics and Readings

Books with Page No.

 

1

What is virus and its economic importance, History of virus and symptomology

Comparative Plant Virology (2nd Ed.,) (3-10, 24)

2

Classification and Replication of plant viruses

 

(Comparative Plant Virology (2nd Ed., (13-17)

3

Replication of RNA and DNA viruses

Comparative Plant Virology (2nd Ed.,) (140,146,147,152,156,157)

4

Physico-chemical properties of plant viruses, Virus-like Pathogens

 

Comparative Plant Virology (2nd Ed.)(50-57)

Plant Viruses: Unique and Intriguing Pathogens,(73-91)

5

Pathological effect of viroids on host plants, Replication of viroid’s

Comparative Plant Virology (2nd Ed.,)(44-45, 46-47)

6

Transmission of plant viruses, Mechanical transmission

 

(PPT slides and handouts)

7

Seed transmission

 

(PPT slides and handouts)

8

Mid term

 

9

Insect transmission

(PPT slides and handouts)

10

Fungal and nematode transmission

(PPT slides and handouts)

11

Beetle and mite transmission

(PPT slides and handouts)

12

Diagnosis of Plant Viruses

(PPT slides and handouts)

13

Viral Diseases

(PPT slides and handouts)

14

Major viral diseases in Pakistan

(PPT slides and handouts)

15

Major viral diseases in Pakistan

(PPT slides and handouts)

16

Final Term

 

Sessional: 20% (Participation, Presentation, Assignment)

Project: -

Presentation: -

Participation: -

Mid Exam: 30%

Final Exam (including practical): 50%

Course Material