UNIVERESITY OF SARGODHA
DEPARTMENT OF BOTANY
Course Title: Plant physiology-I
Credit Hours: 3(2+1)
Instructor: Dr. Ameer Khan
Email: [email protected]
DESCRIPTION
Plant physiology deals with all the internal activities of plants. The subject here to describe plant physiology-I comprises on harvesting of light by plants and its conversion into a chemical energy, mechanism of oxygen evolution by plants, cyclic and non-cyclic electron transport chain. This also gives informations about dark reaction, C3, C4 cycle, mechanisms of photosynthesis in CAM plants and phloem transport. It also develops understanding about acquisition of energy during the process of respiration.
Objectives
- To introduce the concept and importance of plant physiology
- To build a comprehensive understanding of photosynthesis and respiration
- To transfer the basic knowledge of plant physiology, importance of light for living organism, respiration and storage of food in plants
COURSE MATERIAL
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Course Contents |
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Week-1 |
History of photosynthesis, nature and units of light. To determine the volume of CO2 evolved during respiration by plant material. |
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Week-2 |
Determination of oxygenic and anoxygenic photosynthesis, various pigments and photosynthetic activity. To determine the amount of CO2 used by respiring water plant by Winkler Method |
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Week-3 |
Ultrastructure and composition of photo system I and II, Absorption and action spectra of different pigments. |
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Week-4 |
Mechanism of photosynthesis – light absorption, charge separation or oxidation of water, electron and proton transport through thylakoid protein – pigment complexes |
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Week-5 |
Photophosphorylation and its mechanism, To regulate stomatal opening by light of different colors and pH. |
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Week-6 |
CO2 reduction (dark reaction), To extract and separate anthocyanins and other phenolic material from plant material and study their light absorption properties. |
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Week-7 |
C3 pathway and photorespiration, regulation of C3 pathway |
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Week-8 |
C4 pathway and its different form, C3- C4 intermediates |
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Week-9 |
CAM pathway, To categorized C3 and C4 plants through their anatomical and physiological characters |
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Week-10 |
Respiration: synthesis of hexose sugars from reserve carbohydrates, Mechanism of respiration – glycolysis, difference between cytosolic and chloroplatidic glycolysis. |
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Week-11 |
Oxidative decarboxylation, krebs cycle, regulation of glycolysis and krebs cycle |
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Week-12 |
Electron transport and oxidative phosphorylation, aerobic and anaerobic respiration, energetics of respiration |
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Week-13 |
Pentose phosphate pathway, glyoxylate cycle, cyanide resistant respiration. |
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Week-14 |
Translocation of food: pathways of translocation, source and sink interaction, material translocated. |
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Week-15 |
Mechanism of phloem transport, loading and unloading, separation of chloroplast pigments on column chromatogram and their quantification by spectrophotometer. |
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Week-16 |
Leaves and atmosphere: gaseous exchange, mechanism of stomatal regulation, factor affecting stomatal regulation |
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Week-17 |
Assimilation of Nitrogen, Sulphur and phosphorus: The nitrogen cycle, Nitrogen fixation. |
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Week-18 |
Pathways of assimilation of nitrate and ammonium ions, Assimilation of Sulphur and phosphorus. |
Books Related to Plant Physiology-I
- Plant Physiology
Lincoln Taiz and Eduardo Zeiger
7th Edition
- Handbook of Plant and Crop Physiology (Second Edition)
Edited by Mohammad Pessarakli
Marcel Dekker 2001 - Plant Biochemistry, 5rd Edition
Edited by Hans-Walter Heldt
Academic Press - Plant Growth, Hormones, and Development
Edited by Dr. Lalit M. Srivastava
Academic Press 2002
ASSESSMENT CRITEIA
Sessional: 10 (Attendance+ Assignments)
Practical: 15, Mid Term Exam: 30, Final exam: 45.
Learning outcomes
After completion of course work, students will be able to understand main themes of plant physiology at the level of photosynthesis, phloem transport and respiration.
