Plant tissue culture broadly refers to the an in vitro cultivation of plants, seeds and various parts of the plants (organs, embryos, tissues, single cells, protoplasts).With the advances made in the tissue culture technology, it is now possible to regenerate species of any plant in the laboratory.  In this process the growth medium or culture solution is very important as, it is used for growing plant tissue because it contains various plant nutrients in the form of 'jelly' known as agar and plant hormones which are necessary for the growth of plant.

Plant tissue culture is used widely in the plant sciences, forestry, and in horticulture. Applications include: The commercial production of plants used as potting, landscape, and florist subjects, which uses meristem and shoot culture to produce large numbers of identical individuals.

Aims and Objectives

  • To quickly produce mature plants.
  • The production of multiples of plants in the absence of seeds or necessary pollinators to produce seeds.
  • The regeneration of whole plants from plant cells that have been genetically modified.

Assessment criteria:

Sessional (Attendance, assignment & presentation) 10 Marks

Practical Exam           15 Marks

Mid Term Exam         30 Marks

Final Term Marks       45 Marks



Course Material